SOX2 Monoclonal Antibody (20G5), DyLight 550

货号：MA1-014-D550

规格：100 µL

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

抗原：SOX2

物种：人/小鼠

宿主：小鼠

抗体亚型：其它

克隆号：20G5

荧光染料：DyLight 550

类型:	单抗	同型对照：	IgG1, kappa
免疫原性：	Full-length human recombinant protein expressed in bacteria	用法：	1:100(ICC);1:100(IF)

Product Specific InformationMA1-014-D488 has been successfully used in ICC/IF applications with human and mouse samples.MA1-014-D488 can be used for immunofluorescence analysis of LIN28 in human iPSCs.Target InformationSOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SOX2 Antibody (MA1-014-D550) in IFImmunofluorescent analysis of SOX2 (red) showing nuclear staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 550-conjugated SOX2 monoclonal antibody (Product # MA1-014-D550) in 3% BSA-PBS at a dilution of 1:50 and incubated for 1 hour at 37C in the dark. Nuclei (left panel, blue) were stained with DAPI. Images were taken at 60X magnification. SOX2 Antibody (MA1-014-D550) in IFImmunofluorescence analysis of SOX2 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Mouse Monoclonal Antibody (Product # MA1-014-D550) at 5µg/ml in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: red). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: green) was stained with Alexa Fluor™ 488 Phalloidin (Product # A12379, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows SOX2 negative cell line HeLa with no signal. Panel f represents control cells with Isotype control to assess background. The images were captured at 60X magnification.

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