SOX2 Monoclonal Antibody (20G5), HRP

货号：MA1-014-HRP

规格：100 µL

价格：4809

产品类型：流式抗体

品牌：Thermo Fisher

抗原：SOX2

物种：人/小鼠

宿主：小鼠

抗体亚型：其它

克隆号：20G5

荧光染料：HRP

类型:	单抗	同型对照：	IgG1, kappa
免疫原性：	Full-length human recombinant protein expressed in bacteria	用法：	1:250-1:1000(WB)

Product Specific InformationMA1-014-HRP has been successfully used in Western blot with human and mouse samples. Western blot analysis of MA1-014-HRP specifically detects SOX2 protein at ~36 kDa in the nucleus of embryonal carcinoma cells.Target InformationSOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SOX2 Antibody (MA1-014-HRP) in WBWestern blot analysis of SOX2 was performed by loading 50 µg of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated SOX2 monoclonal antibody (Product # MA1-014-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

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