FOXP3 Monoclonal Antibody (FJK-16s), Alexa Fluor 488,

货号：53-5773-82

规格：100 ug

价格：3894

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：其它

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：FJK-16s

荧光染料：Alexa Fluor 488

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor 488
浓度：	0.5 mg/mL	用法：	0.25 µg/test(Flow)

产品详细信息Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.Please note that FJK-16s has been optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (cat. 00-5523). The use of other fixation and staining buffers is not recommended.Applications Reported: This FJK-16s antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This FJK-16s antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

数据

FOXP3 Antibody (53-5773-82) in FlowC57BL/6 mouse splenocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD4 Monoclonal Antibody, APC (Product # 17-0042-82) and 0.125 µg of Mouse IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4724-80) (left) or 0.125 µg of FOXP3 Monoclonal Antibody, Alexa Fluor 488 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Clinical immunology (Orlando, Fla.)Age-dependent loss of tolerance to an immunodominant epitope of glutamic acid decarboxylase in diabetic-prone RIP-B7/DR4 mice.2. Nature communicationsOsteoprotegerin-dependent M cell self-regulation balances gut infection and immunity.3. Cell reportsDifferential Roles of IL-2 Signaling in Developing versus Mature Tregs.4. Bioscience reportsHuai Qi Huang corrects the balance of Th1/Th2 and Treg/Th17 in an ovalbumin-induced asthma mouse model.5. Veterinary immunology and immunopathologySensitive detection of Foxp3 expression in bovine lymphocytes by flow cytometry.

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