PAX6 Polyclonal Antibody

货号：42-6600

规格：100 µg

价格：4809

产品类型：流式抗体

品牌：Thermo Fisher

抗原：PAX6

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：	IgG
免疫原性：	Human Pax6	用法：	1:100(Flow);2.0-3.0 µg/mL(ICC);2.0-3.0µg/mL(IF);1.0-2.0 µg/mL(WB)

Product Specific Information42-6600 has been successfully used in flow cytometry and immunofluorescence analysis of Pax6 in human neural stem cells derived from pluripotent stem cells and reacts with human samples.Target InformationPAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters' anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas.All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

PAX6 Antibody (42-6600) in IFImmunofluorescent analysis of beta-3 Tubulin (green) and PAX6 (red) in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey serum in PBS for 30 min at room temperature. Organoid slices were stained with a Mouse beta-3 Tubulin monoclonal antibody (green; Product # MA1-118) at a dilution of 1:500, and a Rabbit PAX6 polyclonal antibody (red; Product # 42-6600) at a dilution of 1:500 in blocking buffer overnight at 4°C, and then incubated with Donkey anti-Mouse Alexa Fluor 488 (Product # R37114) and Donkey anti-Rabbit Alexa Fluor 568 (Product # A10042) at a dilution of 1:1000 as well as DAPI (blue; 1:25000) in blocking solution at room temperature for 1 hour. Images were taken at 20X magnification. Scale bar: 50 µm. Data courtesy of Dr. Zhexing Wen at Emory University. PAX6 Antibody (42-6600) in IFImmunofluorescent analysis of beta-3 Tubulin (green), PAX6 (red), and CTIP2 (blue) in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey serum in PBS for 30 min at room temperature. Organoid slices were stained with a Mouse beta-3 Tubulin monoclonal antibody (green; Product # MA1-118) at a dilution of 1:500, a Rabbit PAX6 polyclonal antibody (red; Product # 42-6600) at a dilution of 1:500, and a Rat CTIP2 polyclonal antibody (blue) at a dilution of 1:300 in blocking buffer overnight at 4°C, and then incubated with Donkey anti-Mouse Alexa Fluor 488 (Product # R37114), Donkey anti-Rabbit Alexa Fluor 568 (Product # A10042), and Goat anti-Rat Alexa Fluor 350 (Product # A-21093) at a dilution of 1:1000 in blocking solution at room temperature for 1 hour. Images were taken at 20X magnification. Data courtesy of Dr. Zhexing Wen at Emory University. PAX6 Antibody (42-6600) in IHC (P)Immunohistochemistry analysis of PAX6 showing staining in the nucleus of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- PAX6 Polyclonal Antibody (Product # 42-6600) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. PAX6 Antibody (42-6600) in FlowFlow cytometry analysis of Pax6 on human neural stem cells derived from PD-3 iPSCs using Gibco® PSC Neural Induction Medium (Product # A1647801). Cells were fixed, permeabilized, and then stained with a Pax6 polyclonal antibody (Product # 42-6600) at a 1:100 dilution and a Nestin mouse monoclonal antibody (Product # MA1-110) at a 1:100 dilution. After incubation of the primary antibodies for 1 hour on ice, the cells were stained with Alexafluor® 488-conjugated goat anti-rabbit IgG secondary antibody (Product # A-11034) and Alexafluor® 647-conjugated donkey anti-mouse IgG secondary antibody (Product # A-31571) at a dilution of 1:500 for 1 hour on ice. Flow cytometry analysis was performed using the Attune® Acoustic Focusing Cytometer (Product # 4469120). A representative 10,000 cells were acquired for each sample.

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参考文献：

1.Scientific reportsMfn2 is Required for Mitochondrial Development and Synapse Formation in Human Induced Pluripotent Stem Cells/hiPSC Derived Cortical Neurons."42-6600 was used in immunocytochemistry and western blot study mitochondrial development, neurogenesis, and synapse formation in human induced pluripotent stem cell-derived cortical neurons"AuthorsFang D,Yan S,Yu Q,Chen D,Yan SS2.Frontiers in molecular neuroscienceComparison ofin vitroNeuronal Differentiation Capacity Between Mouse Epiblast Stem Cells Derived From Nuclear Transfer and Naturally Fertilized Embryos.3.GliaMicroRNA-365 modulates astrocyte conversion into neuron in adult rat brain after stroke by targeting Pax6.4.eLifeNorrin/Frizzled4 signalling in the preneoplastic niche blocks medulloblastoma initiation.

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