PAX6 Polyclonal Antibody

货号：PA5-85374

规格：100 µL

价格：4806

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：PAX6

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：	IgG
免疫原性：	Recombinant protein encompassing a sequence within the center region of human PAX6.	用法：	1:100-1:1000(ICC);1:100-1:1000(IF);1:500-1:3000(WB)；1:500-1:1000(IP)

Product Specific InformationKeep as concentrated solution.Positive Control: 293T, HeLa, mouse brain, rat brain, PAX6-transfected 293T.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.Target InformationPAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters' anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

PAX6 Antibody (PA5-85374) in IHC (F)Immunohistochemistry analysis of PAX6 in frozen E13.5 rat brain using PAX6 polyclonal antibody (Product # PA5-85374) at a dilution of 1:250. Sample was then incubated with beta Tubulin 3/TUJ1 (red) and DAPI secondary antibody at a dilution of 1:500.PAX6 Antibody (PA5-85374) in IHC (F)Immunohistochemistry analysis of PAX6 in frozen embryonic mouse brain using PAX6 polyclonal antibody (Product # PA5-85374) using 30 µg of sample at a dilution of 1:250.PAX6 Antibody (PA5-85374) in IPImmunoprecipitation analysis of PAX6 in 293T whole cell lysate, A) 293T whole cell lysate, B) control with preimmune rabbit IgG, C) immunoprecipitation of PAX6 protein with PAX6 polyclonal antibody (Product # PA5-85374) using A) 50 µg, B) 2 µg of sample at a dilution of 1:1000. Sample was then incubated with anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.PAX6 Antibody (PA5-85374) in IFImmunocytochemistry analysis of PAX6 in 4% paraformaldehyde-fixed SK-N-SH cells using PAX6 polyclonal antibody (Product # PA5-85374) at a dilution of 1:400. Sample was then incubated with phalloidin secondary antibody at a dilution of 1:50.

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