OCT4 Monoclonal Antibody (GT486)

货号：MA5-31458

规格：100 µL

价格：4806

产品类型：流式抗体

品牌：Thermo Fisher

抗原： OCT4

物种：人/小鼠

宿主：小鼠

抗体亚型：其它

克隆号：GT486

荧光染料：其它

类型:	多抗	同型对照：	IgG2b
免疫原性：	Recombinant protein encompassing a sequence within the center region of human Oct4.	用法：	1:100-1:1000(ICC);1:100-1:1000L(IF);1:500-1:3000(WB)；1:50（Flow）;1:100-1:500(IP)

Product Specific InformationKeep as concentrated solution.Positive Control: NT2D1, human ESC, mouse ESC, HeLa transfected recombinant OCT3/4 protein.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.Target InformationOct4 is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. Oct4 is one of the four Yamaka factors, necessary for embryonic stem cell pluripotency. Pluripotent stem (iPS) cells, can be created by expressing Oct4 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Oct4, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

OCT4 Antibody (MA5-31458) in FlowFlow cytometry analysis of OCT4 in human embryonic stem cells using OCT4 monoclonal antibody (Product # MA5-31458) at a dilution of 1:50. OCT4 Antibody (MA5-31458) in IFImmunocytochemistry analysis of OCT4 in 4% paraformaldehyde-fixed human embryonic stem cells using OCT4 monoclonal antibody (Product # MA5-31458) at a dilution of 1:200. Sample was then incubated with Hoechst secondary antibody. OCT4 Antibody (MA5-31458) in IPImmunoprecipitation analysis of OCT4 in transfected 293T cells, A) cell lysate of transfected 293T cell, B) control with preimmune mouse IgG, C) immunoprecipitation of Oct4 protein with OCT4 monoclonal antibody (Product # MA5-31458) using 2 µg of sample at a dilution of 1:1000. Sample was then incubated with anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE. OCT4 Antibody (MA5-31458) in IHC (P)Immunohistochemistry analysis of OCT4 in paraffin-embedded BT483 xenograft using OCT4 monoclonal antibody (Product # MA5-31458) at a dilution of 1:200.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17927.htm