OCT4 Polyclonal Antibody

货号：PA5-27194

规格：100 µL

价格：4880

产品类型：流式抗体

品牌：Thermo Fisher

抗原：OCT4

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

类型:	多抗	同型对照：	IgG
免疫原性：	Recombinant fragment corresponding to a region within amino acids 159 and 360 of Human OCT3/4	用法：	1:50-1:200(Flow);1:100-1:1000(ICC);1:100-1:1000(IF);1:500-1:3000(WB)

Product Specific InformationPA5-27194 targets OCT3/4 in FACS, IF, ChIP and WB applications and shows reactivity with Human samples.The PA5-27194 immunogen is recombinant fragment corresponding to a region within amino acids 159 and 360 of Human OCT3/4.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.Target InformationThis gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

OCT4 Antibody (PA5-27194) in FlowFlow cytometry analysis of OCT3, 4 in human embryonic stem cells (purple) compared to a rabbit IgG control (black) using an OCT3, 4 polyclonal antibody (Product # PA5-27194) at a dilution of 1:100. OCT4 Antibody (PA5-27194) in IFImmunofluorescent analysis of OCT3, 4 in paraformaldehyde-fixed Human ESC cells using an OCT3, 4 polyclonal antibody (Product # PA5-27194) at a 1:80 dilution. OCT4 Antibody (PA5-27194) in ChIPChromatin immunoprecipitation analysis of OCT3, 4 was performed using cross-linked chromatin from 1x10^6 HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of an OCT3, 4 polyclonal antibody (Product # PA5-27194). Chromatin aliquots from ~1x10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 or exon-2-3 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the rat Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17933.htm