NxSeq® AmpFREE 14000 Low DNA Library Kit 基因文库构建试剂盒

货号：14000-1,14000-2

规格：10 µg,48 rxn

价格：3800,14450

产品类型：二代测序-建库

品牌：Lucigen

The NxSeq AmpFREE Low DNA Library Kit and Adaptors are only compatible with Illumina sequencers.EachNxSeq AmpFREE Low DNA Library Kitcontains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG) and Elution Buffer (EB). Adaptors must be purchased separately.Each box of NxSeq Adaptorscontains (12) different indexed Illumina-compatible adaptors with enough of each adapter for 4 library reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24. NxSeq AmpFREE低DNA文库套件和适配器仅与Illumina测序仪兼容。每个NxSeq AmpFREE低DNA文库套件包含酶混合物（EM），2X缓冲液（2XB），连接酶（LIG）和洗脱缓冲液（EB）。接头必须单独购买。每盒NxSeq接头包含（12）不同的Illumina兼容 index ，每个接头足够4个文库反应。 Box1包含接头1-12，Box2包含接头13-24。

优点：

▪Low Input: Requires as little as 75 ng of sheared input DNA allowing use of limiting samples.- 低输入：需要少至75 ng的剪切输入DNA，允许使用限制性样品。▪High Efficiency:Optimized adaptor ligationproduces more sequenceable fragments in each library, yielding better coverage & depth from single or multiplexed libraries.- 高效：优化的接头连接在每个文库中产生更多可测序的片段，从单个或多重文库中获得更好的覆盖率和深度。▪PCR-free:Prevents the introduction of PCR-bias, providing more uniform coverage.- 无PCR：防止引入PCR偏向，提供更均匀的覆盖。

数据：

图1.Percentage of library DNA with correctly ligated adaptors measured by qPCR.Duplicate libraries were prepped per kit/organism (Human, Staphylococcus aureus, Rhodobacter sphaeroides (1 library only), and E. coli) according to the manufacturer’s recommended input amounts and protocols. Adaptor ligation efficiency was measured by qPCR using the KAPA Library Quantification Kit (Complete ROX Low, cat #KK4873) and matching amplified library as an internal standard.

Library Kit	DNA Input	Total Number of Sequencing Reads Per Library
Staphylococcus aureus	E. coli K12
NxSeq® AmpFREE Low DNA Library Kit	75 ng	5,649,946	4,305,882
Kapa Hyper Prep Kit	250 ng	4,838,726(-15%)	1,647,452(-62%)
Illumina TruSeq DNA PCR-Free Library Prep Kit	1 µg	38,768(-99%)	1,543,558(-64%)

表1.Number of sequencing reads generated per library after multiplexing and running on a MiSeq Instrument.DNA fragment libraries were prepped in parallel for each kit/organism according to the manufacturer’s recommended input amounts and protocols. Libraries were quantitated and normalized to 2 nM using the Bioanalyzer (size) and Qubit Fluorometer (amount). Equimolar amounts of each library were multiplexed and sequenced with a single MiSeq run using 2 ×150 bp chemistry. The number of sequencing reads obtained are shown as well as the percent reduction (%) in total reads compared to the appropriate NxSeq AmpFREE Kit results.

Library Kit	Sample Type	Input Amount	Total Reads	Mapped Reads (repeat masked)
NxSeq® AmpFREE Low DNA Library Kit	Normal gDNA	75 ng	2,163,636	900,338
FFPE DNA	75 ng	1,767,818	688,074
FFPE DNA	150 ng	1,706,714	656,658
Kapa Hyper Prep Kit	Normal gDNA	250 ng	1,567,276(-28%)	650,296(-28%)
FFPE DNA	250 ng	1,270,870(-28%)	487,872(-29%)

表2.Number of sequencing reads generated from matching normal and FFPE gDNA sample libraries.DNA fragment libraries were prepped using the two indicated kits according to the manufacturer’s recommended input amounts and protocols. Libraries were constructed from normal gDNA (Biochain, Cat. No. D1234142-S02) and DNA extracted from a matching FFPE human kidney tissue (Biochain Cat. No. T2234142-S02) using the Qiagen AllPrep DNA/RNA FFPE Kit. The gDNA samples were sheared to ~250 bp before starting library construction. Final libraries were quantitated and normalized to 2 nM using the Bioanalyzer (size) and Qubit Fluorometer (amount). Equimolar amounts of each library were multiplexed and sequenced with a single MiSeq run using 2 × 150 bp chemistry. The number of sequencing reads obtained are shown as well as the percent reduction (%) in total and mapped reads compared to the corresponding NxSeq AmpFREE Kit results using 75 ng of input DNA.

组成成分：

EachNxSeq AmpFREE Low DNA Library Kitcontains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG) and Elution Buffer (EB). Adaptors must be purchased separately.

相关产品：

NxSeq® Adaptors, Box 1（货号#14300-1）NxSeq® Adaptors, Box 2（货号#14400-1）

技术参数

产品优点 - Low Input: Requires as little as 75 ng of sheared input DNA allowing use of limiting samples. - High Efficiency: Optimized adaptor ligation produces more sequenceable fragments in each library, yielding better coverage & depth from single or multiplexed libraries. - PCR-free: Prevents the introduction of PCR-bias, providing more uniform coverage. - Fast: 2 hour, 10 minute protocol saves you time and gets your samples on the sequencer sooner. - Affordable: Best priced and best performing kit available.- 低输入：需要少至75 ng的剪切输入DNA，允许使用限制性样品。 - 高效：优化的接头连接在每个文库中产生更多可测序的片段，从单个或多重文库中获得更好的覆盖率和深度。- 无PCR：防止引入PCR偏向，提供更均匀的覆盖。 - 快速：2小时10分钟的操作可以节省您的时间，并尽快在测序仪上获取样品。 - 经济实惠：价格最优惠，性能最佳的套件。

产品应用 - DNA文库制备

产品类型 建库试剂盒

推荐用于 The NxSeq® AmpFREE Low DNA Library Kit allows you to build the best fragment DNA libraries possible. We optimized each step of the protocol to ensure peak performance on Illumina sequencers. In addition, these kits require only 75 ng of sheared DNA input, and produce libraries in about 2 hours using a streamlined, easy to follow protocol.NxSeq®PampFREE低DNA文库试剂盒允许您构建最佳的片段DNA文库。 我们优化了操作的每个步骤，以确保Illumina测序仪的峰值性能。 此外，这些试剂盒仅需要75 ng的剪切DNA输入，并使用简化的易于遵循的方案，能在约2小时内产生文库。

组成成分 Each NxSeq AmpFREE Low DNA Library Kit contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG) and Elution Buffer (EB). Adaptors must be purchased separately.每个NxSeq AmpFREE低DNA文库试剂盒包含酶混合物（EM），2X缓冲液（2XB），连接酶（LIG）和洗脱缓冲液（EB）。 接头必须单独购买。

辅助产品 NxSeq® Adaptors, Box 1（#14300-1）NxSeq® Adaptors, Box 2（#14400-2）

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