SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), Alexa Fluor 488, eBioscience™

货号：53-8843-42,53-8843-41

规格：100 tests,25 tests

价格：3421,1702

产品类型：流式抗体

品牌：eBioscience

抗原：SSEA4

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

克隆号：eBioMC-813-70 (MC-813-70)

荧光染料：Alexa Fluor 488

类型:	单抗	同型对照：	IgG3
免疫原性：		用法：	5 µL (1 µg)/test(Flow)

Product Specific InformationDescription: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationSSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SSEA4 Antibody (53-8843-42) in FlowStaining of 2102Ep cell line with Mouse IgG3 Isotype Control FITC (Product # 11-4742-42) (open histogram) or Anti-Human SSEA-4 Alexa Fluor® 488 (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.Stem cells and developmentSimplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.2.BloodOvercoming reprogramming resistance of Fanconi anemia cells."53-8843 was used in Immunofluorescence to identify and overcome mechanisms of resistance to the reprogramming of somatic cells defective in the Fanconi anaemia pathway."AuthorsMüller LU,Milsom MD,Harris CE,Vyas R,Brumme KM,Parmar K,Moreau LA,Schambach A,Park IH,London WB,Strait K,Schlaeger T,Devine AL,Grassman E,D'Andrea A,Daley GQ,Williams DA

技术参数

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