Plasmid-Safe™ ATP-Dependent DNase/ATP依赖的DNA酶
货号：E3101K,E3110K
规格：1000 U 10 U/µL,10000 U 10 U/µL
价格：1323,6847
产品类型：核酸酶和抑制剂
品牌：Lucigen
| Plasmid-Safe™ ATP-Dependent DNase selectively hydrolyzes linear double-stranded (ds) DNA to deoxynucleotides at slightly alkaline pH and, with a lower efficiency, linear and closed-circular single-stranded DNAs. The reaction is ATP-dependent, and does not affect closed-circular supercoiled or nicked circular dsDNAs. The enzyme can be conveniently and completely heat-inactivated by a 30 minute incubation at 70°C. Plasmid-Safe DNase is useful as a final “cleanup” of DNA preparations from plasmid and cosmid clones, to avoid the problems caused by contaminating genomic DNA.粘粒、BAC、fosmid和质粒制备物中去除污染的细菌染色体DNA。该酶将从末端逐步降解线性DNA；闭环DNA(例如质粒)不具有游离末端，因此不会被降解。这些特性使得Plasmid-Safe ATP-Dependent DNase成为BAC和fosmid纯化方案的理想选择，例如用于鸟枪测序，以及其他需要高纯度DNA的应用。 |
| 优点： |
| - Digest contaminating linear DNA without digesting nicked or closed-circular dsDNA or supercoiled DNA (plasmids, fosmids, etc.)；- Use as a final purification step for plasmid, cosmid, fosmid, and BAC vector and clone preparations- Utilize whenever protecting circular dsDNA is important - 消化污染的线性DNA，而不消化有缺口或封闭的圆形dsDNA或超螺旋DNA（质粒，粘粒等）；- 用作质粒，cosmid, fosmid，BAC载体和克隆制剂的最终纯化步骤；- 在保护重要环状dsDNA时使用. |
| 组成成分： |
| ▪ Plasmid-Safe™ ATPDependent DNase （10U/μL）：1mL； -20°C▪ Plasmid-Safe™ 10X Buffer：5mL；-20°C▪ ATP Solution (25 mM)：2m L；-20°C |
技术参数 产品优点 - Digest contaminating linear DNA without digesting nicked or closed-circular dsDNA or supercoiled DNA (plasmids, fosmids, etc.)； - Use as a final purification step for plasmid, cosmid, fosmid, and BAC vector and clone preparations - Utilize whenever protecting circular dsDNA is important - 消化污染的线性DNA，而不消化有缺口或封闭的圆形dsDNA或超螺旋DNA（质粒，粘粒等）； - 用作质粒，cosmid, fosmid，BAC载体和克隆制剂的最终纯化步骤； - 在保护重要环状dsDNA时使用.
产品应用 - Plasmid-Safe DNase is useful as a final “cleanup” of DNA preparations from plasmid and cosmid clones, to avoid the problems caused by contaminating genomic DNA. - 去除大规模质粒、粘粒、fosmid和BAC载体或克隆制剂中污染的细菌染色体DNA
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