AmpliScribe™ T7 High Yield Transcription Kit,AmpliScribe™ T7高产转录试剂盒

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AmpliScribe™ T7 High Yield Transcription Kit,AmpliScribe™ T7高产转录试剂盒

货号:AS3107

规格: 50Rxns

价格:5676

产品类型:体外转录试剂盒

品牌:Lucigen

The AmpliScribe™ T7 High Yield Transcription Kit is specially formulated to use high concentrations of NTPs, that can be inhibitory to otherinvitrotranscription systems. AmpliScribe High Yield Transcription reactions can produce >20-fold more full-length RNA transcript than conventionalin vitrotranscription reactions. For example, the AmpliScribe T7 Kit yields approximately 5-7.5 mg/ml of 1.4-kb full-length control RNA in 30-120 minutes, respectively, using 1 µg of template DNA.
优点:
▪ High yield. Produce microgram amounts of RNA from all templates—synthesize both long and short RNA using only one kit.▪ Sensitive. Produce microgram amounts of RNA from as little as 1 ng of template.▪Scaleable. Increase the reaction size to produce milligram amounts of RNA.
组成成分:
▪ AmpliScribe T7 Enzyme Solution▪ 100 mM ATP, CTP, GTP, and UTP Solutions,▪ AmpliScribe 10X Reaction Buffer,▪ 100 mM Dithiothreitol (DTT),▪ RiboGuard™ RNase Inhibitor, ▪ RNase-Free Water, ▪ Control Template DNA and RNase-Free DNase I

技术参数

产品优点 - High yield. Produce microgram amounts of RNA from all templates—synthesize both long and short RNA using only one kit.- Sensitive. Produce microgram amounts of RNA from as little as 1 ng of template.- Scaleable. Increase the reaction size to produce milligram amounts of RNA.- Flexible. Substitute a labeled nucleotide for one of the supplied NTP solutions.- Convenient. Rnase inhibitor included.

产品应用 - High-yield synthesis of RNA from DNA cloned downstream from a T7 RNA polymerase promoter, for microinjection, RNA interference, or antisense experiments.- Structural and functional studies of splicing, processing, heterogeneous nuclear RNAs, tRNAs, viral RNAs, and ribozymes.1- Production of fluorescent2-, biotinylated3-, or digoxigenin4- labeled RNA.

推荐用于 Produce high yields of high quality RNA quickly and easily.

组成成分 AmpliScribe T7 Enzyme Solution, 100 mM ATP, CTP, GTP, and UTP Solutions, AmpliScribe 10X Reaction Buffer, 100 mM Dithiothreitol (DTT), RiboGuard™ RNase Inhibitor, RNase-Free Water, Control Template DNA, and RNase-Free DNase I

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