CRISPRcraft™ S.p. Cas9 Nuclease/CRISPRcraft™Sp Cas9核酸酶（停产））
货号：70020-1,70020-2
规格：120μg,400μg
价格：5503,7125
产品类型：CRISPR基因编辑
品牌：Lucigen
| CRISPRcraft™ S.p. Cas9 Nuclease is a purified recombinant wild-type Streptococcus pyogenes Cas9 nuclease produced in E. coli, containing a C-terminal 6xHis tag and a C-terminal NLS to allow efficient transport to the nucleus. It is provided at high concentration (10 mg/mL, 62 µM) to enable efficient RNP delivery and compatibility with multiple delivery methods including lipid-based transfection and electroporation.CRISPRcraft S.p.Cas9 Nuclease*是在大肠杆菌中生产的纯化的重组野生型化脓性链球菌Cas9核酸酶，其含有C末端6x His标签和C末端核定位序列(NLS)，以使其高效转运至细胞核。它以高浓度(10mg/mL,62pM)提供，以实现有效的核糖核蛋白(RNP)递送和与多种递送方法的相容性，包括基于脂质的转染和电穿孔。 |
| 优点： |
| ▪Recombinant wild-typeStreptococcus pyogenesCas9 nuclease, with NLS;▪Ideal for efficient RNP-based delivery of Cas9/gRNA complexes；▪High concentration (10 mg/mL, 62 µM) compatible with multiple delivery methods.▪带有NLS的重组野生型化脓性链球菌Cas9核酸酶▪ 基于RNP的Cas9 / gRNA复合物高效递送的理想选择▪ 高浓度（10 mg / mL，62 µM）与多种递送方法兼容 |
| 数据： |
| Figure 1.CRISPR RNP delivery.HEK293T cells were transfected in triplicate with RNP containing CRISPRcraft™ S.p. Cas9 Nuclease and guide components from the CRISPRcraft™ S.p. Cas9 Nuclease Control Kit targeting HPRT, using either lipofection or electroporation. Transfection conditions: TransIT-X2® Dynamic Delivery System (Mirus), 50 nM gRNA and 25 nM Cas9; Lipofectamine® RNAiMAX (ThermoFisher Scientific), equimolar Cas9 and gRNA at 25 nM; Ingenio® Electroporation Solution (Mirus), 1.5 µM gRNA and 0.75 µM Cas9, plus Alt-R® Cas9 Electroporation Enhancer (IDT). At 48 hours post-transfection, cleavage efficiency at the HPRT locus was determined using the T7E1 mismatch detection assay and is expressed as % gene modification.图 1.CRISPR RNP传递.将HEK293T细胞用含CRISPRcraft™S.p.的RNP一式三份转染。 CRISPRcraft™S.p.的Cas9核酸酶和导向组件。 使用脂转染或电穿孔靶向HPRT的Cas9核酸酶控制试剂盒。转染条件：TransIT-X2®动态递送系统（Mirus），50 nM gRNA和25 nM Cas9； Lipofectamine®RNAiMAX（ThermoFisher Scientific），等摩尔Cas9和25nM的gRNA; Ingenio®电穿孔溶液（Mirus），1.5 µM gRNA和0.75 µM Cas9，以及Alt-R®Cas9电穿孔增强剂（IDT）。转染后48小时，使用T7E1错配检测测定法确定HPRT基因座处的切割效率，并表示为％基因修饰。Figure 2.In vivogene editing with different guide RNA sequences.HEK293T cells were independently transfected in triplicate with RNP containing CRISPRcraft™ S.p. Cas9 Nuclease and three different guides, each targeting a different loci within human HPRT. Transfections were performed using TransIT-X2® Dynamic Delivery System (Mirus), 20 nM guide and 10 nM Cas9 (2:1 guide:Cas9 molar ratio). Cleavage efficiency at each HPRT locus was determined using the T7E1 mismatch detection assay, 48 hours post-transfection.图2.使用不同的方式RNA序列进行体内基因编辑。用含有CRISPRcraft™S.p.的RNP将HEK293T细胞一式三份独立转染。 Cas9核酸酶和三个不同的方式，每个方式对人类HPRT中的不同基因座。 使用TransIT-X2®动态递送系统（Mirus），20 nM向导和10 nM Cas9（2：1向导：Cas9摩尔比）进行转染。 转染后48小时，使用T7E1错配检测测定法测定每个HPRT基因座处的切割效率。 |
| 相关产品： |
| ▪CRISPRcraft S.p. Cas9 Nuclease Control Kit（货号#70030-1） |
技术参数 产品优点 - Recombinant wild-type Streptococcus pyogenes Cas9 nuclease, with NLS
- Ideal for efficient RNP-based delivery of Cas9/gRNA complexes
- High concentration (10 mg/mL, 62 µM) compatible with multiple delivery methods
- Extensive quality control to ensure activity and purity
- 带有NLS的重组野生型化脓性链球菌Cas9核酸酶
- 基于RNP的Cas9 / gRNA复合物高效递送的理想选择
- 高浓度（10 mg / mL，62 µM）与多种递送方法兼容
- 广泛的质量控制以确保活性和纯度
产品应用 - CRISPRcraft™ S.p. Cas9 Nuclease is a purified recombinant wild-type Streptococcus pyogenes Cas9 nuclease produced in E. coli, containing a C-terminal 6xHis tag and a C-terminal NLS to allow efficient transport to the nucleus. It is provided at high concentration (10 mg/mL, 62 µM) to enable efficient RNP delivery and compatibility with multiple delivery methods including lipid-based transfection and electroporation.
- CRISPR/Cas9基因编辑试验
- 序列特异、RNA介导的dsDNA切割
- 序列富集和去除
产品类型 Gene Editing Nucleases
推荐用于 CRISPRcraft™ S.p. Cas9 Nuclease is a purified recombinant wild-type Streptococcus pyogenes Cas9 nuclease produced in E. coli, containing a C-terminal 6xHis tag and a C-terminal NLS to allow efficient transport to the nucleus. It is provided at high concentration (10 mg/mL, 62 µM) to enable efficient RNP delivery and compatibility with multiple delivery methods including lipid-based transfection and electroporation.
CRISPRcraft™S.p. Cas9核酸酶是在大肠杆菌中生产的纯化的重组野生型化脓性链球菌Cas9核酸酶，包含一个C端6xHis标签和一个C端NLS，可有效转运到细胞核。 它以高浓度（10 mg / mL，62 µM）提供，以实现高效的RNP输送并与多种输送方法兼容，包括基于脂质的转染和电穿孔。
组成成分 CRISPRcraft™ S.p. Cas9 Nuclease contains one C-terminal nuclear localization signal (NLS) sequence and one C-terminal 6xHis tag and is provided as a 10 mg/mL (62 µM) solution or a 3.2 mg/mL (20 µM) solution.
辅助产品 CRISPRcraft S.p. Cas9 Nuclease Control Kit
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