产品优点 CopyControl Fosmid clones grown in TransforMax EPI300 cells can be amplified to 10-50copies per cell. The induction process can be done in any culture volume desired dependingon the needs of the user. Generally, a 1-ml induced culture will provide a sufficient amountof DNA for most applications including sequencing and fingerprinting.

产品应用 The CopyControl™ Induction Solution is used to induce CopyControl clones developedin the CopyControl pCC1 and pCC2 vectors, grown in TransforMax™ EPI300™ E. coli, fromsingle-copy number to high-copy number.The Induction Solution induces expression of a mutant trfA gene contained in theTransforMax EPI300 cells. Expression of trfA gene results in initiation of replication from theoriV high-copy origin of replication and subsequent amplification of the CopyControl clonesand clones developed in the CopyControl pCC1 and pCC2 vectors.The CopyControl Induction Solution can be added to CopyControl clones growingin culture or to agar media prior to plating of CopyControl clones transformed intoTransforMax EPI300 cells.

产品类型 fosmid cloning

推荐用于 The CopyControl™ Induction Solution is used to induce CopyControl clones developedin the CopyControl pCC1 and pCC2 vectors, grown in TransforMax™ EPI300™ E. coli, fromsingle-copy number to high-copy number.

辅助产品 CopyControl™ Fosmid Autoinduction Solution AIS107F -- CopyControl™ Fosmid Library Production Kit CCFOS110 -- CopyControl™ HTP Fosmid Library Production Kit CCFOS059