CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), eBioscience™

货号：14-9987-82,14-9987-80

规格：100 µg,25 µg

价格：1156,0

产品类型：流式抗体

品牌：eBioscience

抗原：CD235a

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：HIR2 (GA-R2)

荧光染料：Purified

类型:	单抗	同型对照：	IgG2b, kappa
浓度：	0.5 mg/mL	用法：	0.1 µg/test(Flow)

Product Specific InformationDescription: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.Applications Reported: HIR2 can be used in flow cytometric analysis.Applications Tested: The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and cell agglutination assay and can be used at approximately 0.1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationGlycophorins A (GPA) and B (GPB) which are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD235a (Glycophorin A) Antibody (14-9987-82)Molecular and cellular biology 2013 - Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 14-9987-82)

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参考文献：

1.Scientific reportsPlatelets confound the measurement of extracellular miRNA in archived plasma.2.Stem cell reportsGenetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.3.British journal of cancerPrognostic significance of CD44 variant 2 upregulation in colorectal cancer."14-9987 was used in Flow cytometry/Cell sorting to investigate the clinical significance of CD133 and CD44 as putative cancer stem cell markers."AuthorsOzawa M,Ichikawa Y,Zheng YW,Oshima T,Miyata H,Nakazawa K,Guan HB,Shiozawa M,Akaike M,Watanabe K,Ota M,Fujii S,Kunisaki C,Ishikawa T,Tanaka K,Akiyama H,Endo I,Taniguchi H4.Epigenetics and chromatinGeneration of bivalent chromatin domains during cell fate decisions."14-9987 was used in Flow cytometry/Cell sorting to investigate the patterns and levels of histone modifications at bivalent genes, showing the generation of bivalent chromatin domains during cell fate decisions."AuthorsDe Gobbi M,Garrick D,Lynch M,Vernimmen D,Hughes JR,Goardon N,Luc S,Lower KM,Sloane-Stanley JA,Pina C,Soneji S,Renella R,Enver T,Taylor S,Jacobsen SE,Vyas P,Gibbons RJ,Higgs DR5.NaturePPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

技术参数

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