CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), PerCP-eFluor 710, eBioscience™
货号：46-9987-42,46-9987-41
规格：100 tests,25 tests
价格：3783,0
产品类型：流式抗体
品牌：eBioscience
抗原：CD235a
物种：人
宿主：小鼠
抗体亚型：IgG2b, kappa
克隆号：HIR2 (GA-R2)
荧光染料：PerCP-eFluor™ 710
类型: | 单抗 | 同型对照： | Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-eFluor 710, eBioscience™ |
浓度： | 5 µL/Test | 用法： | 5 µL (0.002 µg)/test(Flow) |
Product Specific InformationDescription: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analsyis of human whole blood. This can be used at 5 µL (0.002 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationGlycophorins A (GPA) and B (GPB) which are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| CD235a (Glycophorin A) Antibody (46-9987-42) in FlowStaining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD235a (Glycophorin A) PerCP-eFluor® 710 (purple histogram). Non-leukocytes were used for analysis. |
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参考文献： |
| 1.Scientific reportsPlatelets confound the measurement of extracellular miRNA in archived plasma.2.Stem cell reportsGenetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.3.NaturePPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.View published figure on4.Stem cells (Dayton, Ohio)EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.5.The Journal of biological chemistryα-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells. |
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