CD235a (Glycophorin A) Monoclonal Antibody (6A7M), eFluor 450, eBioscience™

货号：48-9884-42

规格：100 tests

价格：2987

产品类型：流式抗体

品牌：eBioscience

抗原：CD235a

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：6A7M

荧光染料：eFluor 450

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test(Flow)

Product Specific InformationDescription: The monoclonal antibody 6A7M recognizes human glycophorin A (also known as CD235a). The antibody reacts with the M allele. Glycophorin A is a 151 amino acid sialoglycoprotein found on the erythrocyte (RBC) and erthyroid progenitor cell membrane at about 500,000 copies per cell. The gene for glycophorin resides on chromosome 4 and has 2 allelic forms: M and N, which differ in two amino acids. The M group possesses Ser1 and Gly5 while the N group has Leu1 and Glu5. Recent data suggest that exposure to toxins can cause mutation or loss of an allele resulting in phenotypic changes. Studies are also beginning to correlate genotype/phenotype with predisposition to cancer and heart disease.Applications Reported: This 6A7M antibody has been reported for use in flow cytometric analysis.Applications Tested: This 6A7M antibody has been pre-titrated and tested bu flow cytometric analysis of human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationGlycophorins A (GPA) and B (GPB) which are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD235a (Glycophorin A) Antibody (48-9884-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD235a (Glycophorin A) PE (Product # 12-9987-82) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human Glycophorin A (M) eFluor® 450 (right). RBC gate used for analysis.

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参考文献：

1.Stem cells translational medicineEnforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

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