EconoTaq PLUS GREEN 2X Master Mix/EconoTaq PLUS GREEN 2X PCR预混液
货号：30033-1 ,30033-2
规格：500 rxns ,1000 rxns
价格：2586,3569
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| EconoTaq PLUS GREEN 2X Master Mix is a ready-to-use PCR master mix containing agarose gel
loading buffer and tracking dyes. A PCR reaction is set up simply by combining the EconoTaq
PLUS GREEN 2X Master Mix with template DNA, primers, and water. After the reaction is
complete, it can be loaded directly onto an agarose gel. All necessary reaction components are
provided in the Master Mix, which contains the following: 0.1 units/µL of EconoTaq DNA
Polymerase, Reaction Buffer (pH 9.0), 400 µM dATP, 400 µM dGTP, 400 µM dCTP, 400 µM dATP,
3 mM MgCl2, and a proprietary mix of PCR Enhancer/Stabilizer and blue and yellow tracking dyes.
For difficult templates, such as those containing GC content up to 75%, it has been found that
EconoTaq PLUS GREEN 2X Master Mix performs well with denaturation temperatures up to 98 °C. EconoTaq PLUS GREEN 2X预混液是一种即用型PCR预混液，其中包含琼脂糖凝胶上样缓冲液和跟踪染料。 只需将EconoTaq PLUS GREEN 2X Master Mix与模板DNA，引物和水混合即可设置PCR反应。 反应完成后，可以将其直接上样到琼脂糖凝胶上。 预混液中提供了所有必需的反应组分，其中包括：0.1单位/ µL EconoTaq DNA聚合酶，反应缓冲液（pH 9.0），400 µM dATP，400 µM dGTP，400 µM dCTP，400 µM dATP，3 mM MgCl2，以及PCR增强剂/稳定剂与蓝色和黄色跟踪染料的专有混合物。 对于困难的模板（例如那些GC含量高达75％的模板），发现EconoTaq PLUS GREEN 2X Master Mix在变性温度高达98°C时表现良好。 |
| 优点： |
| ▪ Outstanding performance and value are perfect for routine PCR；▪Can be cycled up to 98°C to amplify the most challenging GC-rich templates others cannot；▪Ready-to-use PCR reaction master mixes, contain dNTPs and PCR Enhancer. ▪出色的性能和价值非常适合常规PCR； ▪可以在高达98°C的温度下循环，以扩增其他人无法解决的最具挑战性的富含GC的模板。▪非校对聚合酶 |
| 数据： |
| Figure 1.EconoTaq PLUS GREEN Master Mix vs. GoTaq® Green master mix (Promega) in PCR. PCR was performed according to the manufacturer’s recommendations using primers specific for the indicated genes.Panel A,0.7 kb prokaryotic single-stranded DNA binding protein (genomic DNA);Panel B,2.7 kb prokaryotic DNA polymerase A (genomic DNA);Panel C,4.5 kb gene (plasmid DNA).图 1. PCR中的EconoTaq PLUS GREEN预混液与GoTaq®Green预混液（Promega）。 根据制造商的建议，使用针对所示基因的特异性引物进行PCR。 图A，0.7kb原核单链DNA结合蛋白（基因组DNA）； 图B，2.7kb的原核DNA聚合酶A（基因组DNA）； 图C，4.5kb基因（质粒DNA）。Figure 2.GC-rich regions from human genomic DNA were PCR amplified using 98° C denaturation.图 2.使用98°C变性PCR扩增了来自人类基因组DNA的富含GC的区域。Figure 3.Easy visualization with EconoTaq PLUS GREEN. During electrophoresis, the green loading color separates into a slow-moving blue dye and a fast-moving yellow dye.图 3.使用EconoTaq PLUS GREEN轻松可视化。 电泳期间，绿色加载颜色分为缓慢移动的蓝色染料和快速移动的黄色染料。 |
技术参数 产品优点 - Outstanding performance and value are perfect for routine PCR. - Can be cycled up to 98°C to amplify the most challenging GC-rich templates others cannot. - Ready-to-use PCR reaction master mixes, contain dNTPs and PCR Enhancer. - Non-proofreading polymerase - EconoTaq PLUS GREEN contains tracking dyes for gel electrophoresis- 出色的性能和价值非常适合常规PCR。- 可以在高达98°C的温度下循环，以扩增其他人无法解决的最具挑战性的富含GC的模板。- 非校对聚合酶- 即用型PCR反应预混合物，包含dNTP和PCR增强剂。- EconoTaq PLUS GREEN包含用于凝胶电泳的跟踪染料
产品应用 - Use EconoTaq PLUS GREEN 2X Master Mix when the PCR products will be analyzed by agarose gel electrophoresis and ethidium bromide staining.- 当将通过琼脂糖凝胶电泳和溴化乙锭染色分析PCR产物时，请使用EconoTaq PLUS GREEN 2X Master Mix。
推荐用于 Routine PCR
组成成分 EconoTaq PLUS GREEN 2X Master Mix contains all of the components necessary toperform PCR amplification, except for template and primers. The Master Mix also contains agarose gelloading buffer and tracking dyes.
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