Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Alexa Fluor 700, eBioscience™

货号：56-5981-82,56-5981-80

规格：100 µg,25 µg

价格：2947,1474

产品类型：流式抗体

品牌：eBioscience

抗原：Sca-1

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：D7

荧光染料：Alexa Fluor 700

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor 700, eBioscience™
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

Product Specific InformationDescription: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationLy-6A/E molecules are implicated in the regulation of T cell activation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Ly-6A/E (Sca-1) Antibody (56-5981-82) in FlowStaining of 3-day Con A-stimulated C57BL/6 splenocytes with 0.25 µg of Rat IgG2a K Isotype Control Alexa Fluor® 700 (Product # 56-4321-80) (open histogram) or 0.25 µg of Anti-Mouse Ly-6A/E (Sca-1) Alexa Fluor® 700 (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.eLifeDifferential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.2.Nature communicationsStem cell factor is selectively secreted by arterial endothelial cells in bone marrow.3.Cell stem cellLarge-Scale Clonal Analysis Resolves Aging of the Mouse Hematopoietic Stem Cell Compartment."56-5981 was used in Flow cytometry/Cell sorting to determine the age-related functional changes to the haematopoietic stem cell compartment."AuthorsYamamoto R,Wilkinson AC,Ooehara J,Lan X,Lai CY,Nakauchi Y,Pritchard JK,Nakauchi H4.The Journal of investigative dermatologySpatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations."56-5981 was used in Flow cytometry/Cell sorting to suggest that ex vivo expansion or in vivo ablation of specific fibroblast subpopulations may have therapeutic applications in wound healing and diseases characterized by excessive fibrosis."AuthorsPhilippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM5.PloS oneZoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB.

技术参数

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