Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PerCP-Cyanine5.5, eBioscience™

货号：45-5981-82,45-5981-80

规格：100 µg,25 µg

价格：2534,1038

产品类型：流式抗体

品牌：eBioscience

抗原：Sca-1

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：D7

荧光染料：PerCP-Cy5.5

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PerCP-Cyanine5.5, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25 µg/test(Flow)

Product Specific InformationDescription: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationLy-6A/E molecules are implicated in the regulation of T cell activation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Ly-6A/E (Sca-1) Antibody (45-5981-82) in FlowStaining of C57Bl/6 bone marrow cells with Mouse Hematopoietic Lineage eFluor® 450 Cocktail (Product # 88-7772-72), Anti-Mouse CD117 (c-Kit) APC (Product # 17-1171-82) and 0.125 µg of Anti-Mouse Ly-6A/E (Sca-1) PerCP-Cyanine5-5 (right). Lineage negative/low, viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), were used for analysis.

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参考文献：

1.Epigenetics and chromatinEstablishment of regulatory elements during erythro-megakaryopoiesis identifies hematopoietic lineage-commitment points.AuthorsHeuston EF,Keller CA,Lichtenberg J,Giardine B,Anderson SM,Hardison RC,Bodine DM2.Cell stem cellLarge-Scale Clonal Analysis Resolves Aging of the Mouse Hematopoietic Stem Cell Compartment."45-5981 was used in Flow cytometry/Cell sorting to determine the age-related functional changes to the haematopoietic stem cell compartment."AuthorsYamamoto R,Wilkinson AC,Ooehara J,Lan X,Lai CY,Nakauchi Y,Pritchard JK,Nakauchi H3.Arteriosclerosis, thrombosis, and vascular biologyAdventitial Sca1+ Cells Transduced With ETV2 Are Committed to the Endothelial Fate and Improve Vascular Remodeling After Injury."45-5981 was used in Flow cytometry/Cell sorting to provide evidence that the genetic manipulation of vascular progenitors is a promising approach to improve vascular function after endothelial injury."AuthorsLe Bras A,Yu B,Issa Bhaloo S,Hong X,Zhang Z,Hu Y,Xu Q4.Nature communicationsIn utero nanoparticle delivery for site-specific genome editing.AuthorsRicciardi AS,Bahal R,Farrelly JS,Quijano E,Bianchi AH,Luks VL,Putman R,López-Giráldez F,Coşkun S,Song E,Liu Y,Hsieh WC,Ly DH,Stitelman DH,Glazer PM,Saltzman WM5.The Journal of clinical investigationFOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging.

技术参数

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