Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-eFluor 610, eBioscience™

货号：61-5981-82,61-5981-80

规格：100 µg,25 µg

价格：3481,0

产品类型：流式抗体

品牌：eBioscience

抗原：Sca-1

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：D7

荧光染料：PE-eFluor 610

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor 610, eBioscience™
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

Product Specific InformationDescription: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationLy-6A/E molecules are implicated in the regulation of T cell activation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Ly-6A/E (Sca-1) Antibody (61-5981-82) in FlowStaining of C57Bl/6 bone marrow cells with Mouse Hematopoietic Lineage Cocktail eFluor® 450 (Product # 88-7772-72), Anti-Mouse CD117 (c-Kit) APC (Product # 17-1171-82), and 0.25 µg of Rat IgG2a K Isotype Control PE-eFluor® 610 (Product # 61-4321-82) (left) or 0.25 µg of Anti-Mouse Ly-6A/E (Sca-1) PE-eFluor® 610 (right). Lineage negative/low viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.

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参考文献：

1.eLifeDifferential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.2.Nature communicationsStem cell factor is selectively secreted by arterial endothelial cells in bone marrow.3.PloS oneZoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB.4.The Journal of clinical investigationFOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging.5.Nature communicationsInduced p53 loss in mouse luminal cells causes clonal expansion and development of mammary tumours.

技术参数

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