Rat IgG2a kappa Isotype Control (eBR2a), Biotin

货号：13-4321-82,13-4321-81,13-4321-85

规格：100 µg,50 µg,500 µg

价格：1177,640,2900

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBR2a

荧光染料：Biotin

类型:	大鼠同型对照	同型对照：	IgG2a, kappa
浓度：	0.5 mg/mL	用法：	Assay-Dependent

Product Specific InformationDescription: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, flow cytometric analysis, and ELISA.Applications Tested: This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody.Filtration: 0.2 µm post-manufacturing filtered.Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)Alendronate attenuates eosinophilic airway inflammation associated with suppression of Th2 cytokines, Th17 cytokines, and eotaxin-2."13-4321 was used in Flow cytometry/Cell sorting to examine the effect of alendronate, a nitrogen-containing bisphosphonate, on allergic airway inflammation in a mouse model."2.NPJ vaccinesEnhancing vaccine antibody responses by targeting Clec9A on dendritic cells."13-4321 was used in Flow cytometry/Cell sorting to identify that Clec9A-targeted priming, conventional boosting strategy, M2e immunisation protected mice from infection with lethal doses of influenza H1N1 virus."3.Journal of cellular physiologyCross-circulation and cell distribution kinetics in parabiotic mice."13-4321 was used in Flow cytometry/Cell sorting to conclude that parabiosis can provide important insights into defining tissue distribution, residence times, and recirculating pools."4.Nature medicineThe pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis."13-4321 was used in Functional assays to elucidate the mechanisms by which glycocalyx loss occurs during sepsis, and how this allows for neutrophil adhesion within the pulmonary circulation."

技术参数

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