LavaLAMP™ RNA Component Kit
货号：30096-1,30097-1
规格：500 rxn ,with Dye，500 rxn
价格：3592,3937
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| Like the related,LavaLAMP™ RNA Master Mix, this component kit is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. With these unique features, it is possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) using a single DNA polymerase. This enzyme first copies the RNA into cDNA, and then loop mediated isothermal amplification takes over to amplify the initial RNA target sequence present in the new cDNA making amplified and detectable target-specific, dsDNA. |
| 优点： |
| ▪Isothermal Amplification:Enables running amplification reactions using less complex and lower cost instruments；▪Lyophilization-ready:All kit components are lyophilization compatible, thus avoiding redesign of assays to remove components known to inhibit lyophilization；▪ Fully Optimizable:Non-master mix kit format gives you complete control of reaction formulation to maximize assay performance； |
| 数据： |
| Figure 1. Comparing the performance of the LavaLAMP™ RNA Component Kit and the LavaLAMP™ RNA Master Mix.LAMP reactions (6 replicates per test) were set up using the indicated kits and targets (A, MS2 and B, Zika) with the same concentrations of Target-Specific Primer Mixes (1X) and the same target input amounts. The LavaLAMP RNA Component Kit reactions were formulated to 1X LavaLAMP RNA Buffer, 5 mM MgSO4 and 0.8 mM dNTPs (each) to match the final formulations of the LavaLAMP RNA Master Mix reactions. Green Fluorescent Dye was included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at 68°C for both the MS2 and Zika targets and fluorescence was measured during the course of the 60-minute reactions to determine TTR. NTC denotes No Target Control. |
| 组成成分： |
| ▪10X LavaLAMP™ RNA Buffer：1.25 mL▪LavaLAMP™ RNA Enzyme : 500 µL▪Magnesium Sulfate, 100 mM：1.5 mL▪RNA Positive Control LAMP Primer Mix：25 µL▪RNA Positive Control：10 µL▪Green Fluorescent Dye：500 µL |
| 相关产品： |
| ▪Green Fluorescent Dye（货号#30078-1） |
技术参数 产品优点 - Isothermal Amplification: Enables running amplification reactions using less complex and lower cost instruments - Lyophilization-ready: All kit components are lyophilization compatible, thus avoiding redesign of assays to remove components known to inhibit lyophilization - Fully Optimizable: Non-master mix kit format gives you complete control of reaction formulation to maximize assay performance
产品应用 - Sensitive, Fast RNA Detection with this Fully Optimizable RNA LAMP Kit
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