NxGen® phi29 DNA Polymerase/NxGen® phi29 DNA聚合酶
货号：30221-1,30221-2
规格：2000 U ,10000 U
价格：2343,9179
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| Phi29 DNA Polymerase is normally responsible for the replication of the Bacillus Subtilis phage phi29 (1).The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity (2) and a 3′→ 5′ proofreading exonuclease function (3). The NxGen® phi29 DNA Polymerase is often used for whole genome amplification or wherever a DNA polymerase with strong strand displacement activity is required. Phi29 DNA聚合酶通常负责枯草芽孢杆菌噬菌体phi29（1）的复制。该酶是一种高度加工的DNA聚合酶（每个结合事件最多可插入70,000个碱基），具有强大的链置换活性（2）和3' →5'校对核酸外切酶功能（3）。 NxGen®phi29 DNA聚合酶通常用于全基因组扩增或需要具有强链置换活性的DNA聚合酶。NxGen phi29 DNA聚合酶（Φ29 DNA聚合酶）是一种具有连续合成能力及优越置换活性的酶。该酶还含有3’→5’核酸外切酶活性，具有校对能力。 |
| 优点： |
| ▪ Excellent strand displacement ;▪ Fidelity;▪ Speed. |
| 数据： |
| Figure 1. Whole Genome Amplification with NxGen® phi29 DNA Polymerase. 1 ng of human genomic DNA was amplified by whole genome amplification as outlined in Protocol 2 of this manual. Five different lots of NxGen phi29 DNA Polymerase were used and duplicate NTC and (+) human genomic amplification图1.使用NxGen®phi29 DNA聚合酶进行全基因组扩增。 如本手册的方案2所述，通过全基因组扩增来扩增1 ng人类基因组DNA。 使用了五批不同的NxGen phi29 DNA聚合酶，并重复了NTC和（+）人类基因组扩增Figure 2. Whole genome amplification with NxGen® phi29 DNA Polymerase using 16 hour reaction incubations. 1 ng of human genomic DNA was amplified by whole genome amplification as outlined in Protocol 2 of this manual. Five different lots of NxGen phi29 DNA Polymerase were used and duplicate NTC and (+) human genomic amplification reactions were set up. Amplification reactions were incubated for 16 hours at 30°C and products were analyzed in a 0.7% agarose gel and stained with ethidium bromide to visualize amplified DNA. The red arrows indicate NTC reactions with detectable background (input template independent) amplification. reactions were set up. Amplification reactions were incubated for 4 hours at 30°C and products were analyzed in a 0.7% agarose gel and stained with ethidium bromide to visualize amplified DNA. 图2.使用NxGen®phi29 DNA聚合酶进行16小时反应孵育的全基因组扩增。 如本手册的方案2所述，通过全基因组扩增来扩增1 ng人类基因组DNA。 使用了五个不同批次的NxGen phi29 DNA聚合酶，并建立了重复的NTC和（+）人类基因组扩增反应。 将扩增反应在30°C下孵育16小时，然后在0.7％的琼脂糖凝胶中分析产物，并用溴化乙锭染色以观察扩增的DNA。 红色箭头表示具有可检测背景（独立于起始模板）扩增的NTC反应。 建立了反应。 将扩增反应在30°C下孵育4小时，然后在0.7％的琼脂糖凝胶中分析产物，并用溴化乙锭染色以观察扩增的DNA。Figure 3. Whole genome amplification with NxGen® phi29 DNA Polymerase using the pre-incubation cleanup step and a 16 hour incubation time. 1 ng of human genomic DNA was amplified by whole genome amplification as outlined in Protocol 1 of this manual. Lot 4 of NxGen phi29 DNA Polymerase was used to set up triplicate NTC and (+) human genomic amplification reactions. Reactions were pre-incubated in the absence of target DNA as outlined in Protocol 1 of this manual followed by incubation for 16 hours at 30°C. Products were analyzed in a 0.7% agarose gel and stained with ethidium bromide to visualize amplified DNA. Note the lack of background amplification in the NTC lanes compared to the background amplification observed for Lot 4 in Figure 2. 图3.使用预孵育清理步骤和16小时孵育时间，使用NxGen®phi29 DNA聚合酶进行的全基因组扩增。 如本手册的方案1所述，通过全基因组扩增可扩增1 ng人类基因组DNA。 NxGen phi29 DNA聚合酶的批次4用于建立一式三份的NTC和（+）人类基因组扩增反应。 如本手册方案1所述，将反应液在不存在目标DNA的情况下进行预孵育，然后在30°C孵育16小时。 在0.7％琼脂糖凝胶中分析产物，并用溴化乙锭染色以可视化扩增的DNA。 请注意，与图2中批次4观察到的背景扩增相比，NTC泳道中没有背景扩增。 |
| 组成成分： |
| ▪NxGen® phi29 DNA Polymerase：储存在-20°C▪10X phi29 DNA Polymerase Buffer：储存在-20°C |
| 相关产品： |
▪2.5 mM dNTP Mix（#30030） |
| 参考文献： |
| 1. Blanco, L. and Salas, M. (1984) Proc. Natl. Acad. Sci. USA, 81, 5325-5329. 2. Blanco, L. et al. (1989) J. Biol. Chem., 264, 8935-8940. 3. Garmendia, C. et al. (1992) J. Biol. Chem., 267, 2594-2599. |
技术参数 产品优点 - Excellent strand displacement, fidelity and speed. - 出色的链置换能力，保真度和速度
产品应用 - The NxGen® phi29 DNA Polymerase is often used for whole genome amplification or wherever a DNA polymerase with strong strand displacement activity is required. - NxGen®phi29DNA聚合酶通常用于全基因组扩增或需要具有强链置换活性的DNA聚合酶的任何地方。 - 可用于单细胞测序的DNA聚合酶。
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