BigEasy v2.0 Linear Cloning Kit (pJAZZ-OC NotI Vector)，BigEasy v2.0线性克隆试剂盒
货号：43024-1,43024-2,43024-3
规格：5 rxns,10 rxns,20 rxns
价格：4752,8048,12960
产品类型：二代测序-建库
品牌：Lucigen
| The BigEasy Kit with the pJAZZ vector is ideal for constructingbias-free, large-insert genomic libraries, or for cloning difficult DNA of any size up to 30 kb. Because the novel pJAZZ vector is maintained as a linear molecule, the ends of the vector can rotate freely. As a result, the vector does not supercoil. Without the torsional stress induced by supercoiling, otherwise unclonable sequences (e.g., repetitive, or A/T-rich or G/C-rich sequences) are stabilized. The pJAZZ linear cloning vector is maintained at low copy number (5-10/cell) inBigEasy-TSA™ Electrocompetent Cells(>4 × 1010cfu/µg), which are required for transformation and propagation. The copy number can be induced 5-10X in this strain. In addition, the pJAZZ vector incorporates Lucigen’s CloneSmart® technology for transcription-free cloning, which further increases insert stability.pJAZZ clones can be isolated using conventional plasmid prep methods and sequenced using standard techniques. |
| 优点： |
| ▪ Maximum insert stability；▪Efficiently clone any insert up to 30 kb；▪Create libraries from A/T-rich or G/C-rich genomes； |
| 数据： |
| Figure 1.pJAZZ-OC and pJAZZ-OK linear vectors. RepA, replication factor and low copy origin of replication (~2-4 per cell; inducible 5-10 fold); Camr - chloramphenicol resistance gene; Kanr - kanamycin resistant gene; telN - protelomerase gene; cB - replication regulator. Approximate positions of transcription terminators (T) are indicated.Figure 2.15-20 kb PCR amplification products cloned into the pJAZZ® linear vector.Figure 3.Mouse genomic DNA sheared to6-20 kb and cloned into the pJAZZ linear vector. |
| 相关产品： |
| ▪BigEasy® v2.0 Linear Clon Kit (pJAZZ-OC Blunt Vector)（货号#43018）▪ BigEasy® v2.0 Linear Clon Kit (pJAZZ-OK Blunt Vector)（货号#43036）▪ BigEasy® v2.0 Linear Clon Kit (pJAZZ-OK NotI Vector)（货号#43042）▪BigEasy-TSA Electrocompetent Cells（货号#60224） |
技术参数 产品优点 - Maximum insert stability. - Efficiently clone any insert up to 30 kb. - Create libraries from A/T-rich or G/C-rich genomes. - Clone gene clusters or operons. - Inducible copy number.
产品应用 - The BigEasy Kit with the pJAZZ vector is ideal for constructing bias-free, large-insert genomic libraries, or for cloning difficult DNA of any size up to 30 kb
组成成分 BigEasy v2.0 Linear Cloning Kits include: Dephosphorylated pJAZZ vector pre-cut at either a SmaI (blunt) or NotI site, CloneSmart DNA Ligase, CloneDirect™ 10X Ligation Buffer (includes ATP), Sequencing Primers, Positive Control Insert DNA, DNA Terminator® End Repair Enzyme, BigEasy-TSA Electrocompetent Cells in SOLO packaging (1 transformation per tube), Recovery Medium, Transformation Control DNA, and complete protocols.
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