Mouse IgG2b kappa Isotype Control (eBMG2b), Functional Grade, eBioscience™/Mouse IgG2b kappa同型对照(eBMG2b), 功能抗体, eBioscience™

货号：16-4732-82,16-4732-85,16-4732-38,16-4732-025,16-4732-050

规格：100 µg,500 µg,5 mg,25 mg,50 mg

价格：1156,2103,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：eBMG2b

荧光染料：其它

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类型:	同型对照(功能抗体）	同型对照：	IgG2b, kappa
浓度：	1 mg/mL	用法：	Assay-Dependent（Flow）；Assay-Dependent（对照）；Assay-Dependent（FN）

产品详细信息Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.Applications Reported: This mouse IgG2b isotype control has been reported for use in flow cytometric analysis.Applications Tested: This mouse IgG2b Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

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参考文献：

流式1.mAbsMonoclonal antibodies specific to human Δ42PD1: A novel immunoregulator potentially involved in HIV-1 and tumor pathogenesis."16-4732 was used in Flow cytometry/Cell sorting to generate high-affinity mAbs from δ42PD1-immunised mice, that can be used to further investigate the role of δ42PD1 in tumour pathogenesis."AuthorsCheng L,Tang X,Liu L,Peng J,Nishiura K,Cheung AK,Guo J,Wu X,Tang HY,An M,Zhou J,Cheung KW,Wang H,Guan X,Wu Z,Chen Z2.Journal of immunology (Baltimore, Md. : 1950)Soluble CD80 restores T cell activation and overcomes tumor cell programmed death ligand 1-mediated immune suppression."16-4732 was used in Flow cytometry/Cell sorting to identify CD80-Fc as an alternative and potentially more efficacious therapeutic agent for overcoming PDL1-induced immune suppression and facilitating tumor-specific immunity."AuthorsHaile ST,Dalal SP,Clements V,Tamada K,Ostrand-Rosenberg S

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