MMLV High Performance Reverse Transcriptase MMLV高效逆转录酶
货号：RT80125K
规格：25000 units
价格：2282
产品类型：反转录酶
品牌：Lucigen
| MMLV High Performance Reverse Transcriptase (MMLV HP RT) is highly efficient at producing full-length cDNA from RRNA Templates at least 15 kb. MMLV HP RT demonstrates significantly more activity than other MMLV Reverse transcriptase enzymes. It is capable of producing cDNA from as little as 100 pg of total RNA for real-time RT-PCR (qRTPCR) analysis and other applications. MMLV HP RT is supplied at a concentration of 200 U/μl. An MMLV HP RT 10X Reaction Buffer that provides for optimal activity and 100 mM dithiothreitiol (DTT) are also provided. MMLV高效逆转录(MMLV HP Rt) 显示出比其他市售MMLV RT酶显著更高的逆转录酶活性。通常，与来自许多其他供应商的200单位MMLV RT酶相比，全长cDNA合成仅需要100单位的MMLV HP RT酶。该酶可从长度超过15kb的RNA模板合成全长cDNA，起始RNA的量可从皮克级开始。 |
| 组成成分： |
| ▪ RT80125K：储存在-20°C▪ MMLV High Performance Reverse Transcriptase ：储存在-20°C▪ MMLV HP RT 10X Reaction Buffer ：储存在-20°C▪ 100 mM DTT：储存在-20°C |
| 相关产品： |
| ▪Sterile Nuclease-Free Water（#RT80125K） |
技术参数 产品应用 - Synthesize full-length cDNA (>15 kb)
- Amplify first-strand cDNA from picogram amounts of total RNA
- Optimize the RT reaction to your specific needs by using the first strand cDNA synthesis primers, dNTPs and RNase inhibitor of your choice (not included).
- 从总RNA或poly(A)富集的RNA合成cDNA，用于随后的PCR、qPCR或RNA-Seq文库制备。
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