RNA 5' Polyphosphatase/RNA 5'多磷酸酶
货号：RP8092H
规格：200 U
价格：2040
产品类型：Modifying Enzymes连接酶
品牌：Lucigen
| RNA 5′ Polyphosphatase* is a Mg2+-independent, 19,000-kDa phosphohydrolase. The enzyme sequentially removes the γ and β phosphates from 5′-triphosphorylated RNA (primary RNA transcripts) and 5′-diphosphorylated RNA. 5′ pppN--------OH 3′ or 5′ ppN--------OH 3′ ↓ 5′ pN------------OH 3′ + 2 PiRNA 5′ Polyphosphatase has no activity on RNA with a 5′ cap (e.g., 5′ m7GpppN-----OH 3′), a 5′-monophosphorylated end (5′ pN-----OH 3′), or a 5′-hydroxyl end (5′ HO-----OH 3′). However, NTPs and dNTPs are used as substrates yielding the corresponding NMPs and dNMPs + inorganic phosphate. (d)NTP → (d)NMP + 2 Pi RNA 5′ Polyphosphatase is available in a 200 Unit size at a concentration of 20 U/µL. A 10X Reaction buffer is provided with the enzyme.RNA 5，多聚核酸酶是一种Mg2+非依赖性磷酸水解酶。它从5，-三磷酸化的RNA和 5′-二磷酸化的 RNA.（如初级RNA转录本）上依次去除γ和β 磷酸；但它不会使单磷酸化或5，-帽结构的RNA去磷酸化。5，pppN—OH 3，→5，pN—OH 3，+2P。 |
| 优点： |
| ▪ Specific: Removes the γ and β phosphates from 5´-triphosphorylated and the β phosphates from diphosphorylated RNA, but will not dephosphorylate monophosphorylated or 5´-capped RNA▪ Heat Inactivated: Heat at 65°C for 20 minutes to kill enzyme activity▪ Flexible: Use for various applications such as 5´-RNA ligation-tagging using T4 RNA Ligase, analysis of 5´-end structure of RNA, and preparation of substrate RNAs for subsequent degradation with Terminator™ 5´-Phosphate-Dependent Exonuclease ▪ 特异性：从5′-三磷酸化的RNA中去除了γ和β磷酸，从二磷酸化的RNA中去除了β磷酸，但不会使单磷酸化或5′-加帽的RNA去磷酸化▪ 加热灭活：在65°C加热20分钟以杀死酶活性▪ 灵活：可用于多种应用，例如使用T4 RNA连接酶进行5´-RNA连接标记，分析RNA 5´末端结构以及制备底物RNA，随后用Terminator™5´-磷酸盐依赖性核酸外切酶降解 |
| 组成成分： |
| ▪ RNA 5′ Polyphosphatase (20 Unit/µL)：储存在-20℃▪ RNA 5′ Polyphosphatase 10X Reaction Buffer：储存在-20℃ |
| 相关产品： |
▪Riboguard™ RNase Inhibitor（#RG90910K/RG90925） ▪Sterile Nuclease-Free Water（# W7350ML） |
技术参数 产品优点 - Specific: Removes the γ and β phosphates from 5´-triphosphorylated and the β phosphates from diphosphorylated RNA, but will not dephosphorylate monophosphorylated or 5´-capped RNA
- Heat Inactivated: Heat at 65°C for 20 minutes to kill enzyme activity
- Flexible: Use for various applications such as 5´-RNA ligation-tagging using T4 RNA Ligase, analysis of 5´-end structure of RNA, and preparation of substrate RNAs for subsequent degradation with Terminator™ 5´-Phosphate-Dependent Exonuclease
- 特异性：从5′-三磷酸化的RNA中去除了γ和β磷酸，从二磷酸化的RNA中去除了β磷酸，但不会使单磷酸化或5′-加帽的RNA去磷酸化
- 加热灭活：在65°C加热20分钟以杀死酶活性
- 灵活：可用于多种应用，例如使用T4 RNA连接酶进行5´-RNA连接标记，分析RNA 5´末端结构以及制备底物RNA，随后用Terminator™5´-磷酸盐依赖性核酸外切酶降解
产品应用 - Conversion of 5′-triphosphorylated RNA to 5′-monophosphorylated RNA for use in 5′-T4 RNA Ligase-mediated RNA “tagging” strategies.
- Analysis of 5′-end structure of RNA.
- Preparation of substrate RNA molecules for subsequent degradation using Terminator™ 5′-Phosphate-Dependent Exonuclease (Lucigen).
- 将5-三磷酸化RNA转化为5，-单磷酸化RNA，用于T4 RNA连接酶的5'-RNA连接标记方法
- RNA 5，末端结构分析
- 制备底物RNA分子用于后续的Terminator Exonuclease降解反应
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-18222.htm
QQ:1749072012