Expresso® Solubility and Express Screening System/Expresso®溶解度和表达筛选系统
货号：49060-1
规格：24 rxns
价格：11152
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| The Expresso® Solubility and Expression Screening System is an all-in-one system that enables PCR amplification, rapid cloning, and expression of your protein of interest with a diverse panel of powerful and cleavable solubility tags. Lucigen has identified 7 unique and literature-validated solubility tags and combined them with Expr essioneering™ technology to create a suite of cloning-ready vectors for rapid and parallel evaluation of your protein with all 7 solubility tags (plus a 6xHis-tagged control vector). Expresso®溶解度和表达筛选系统是一种多合一系统，可通过各种功能强大且可裂解的溶解度标签进行PCR扩增，快速克隆和表达您感兴趣的蛋白质。 Lucigen已经鉴定出7种独特且经过文献验证的溶解度标签，并将它们与Expr essioneering™技术结合使用，以创建一套可克隆的载体，用于通过所有7种溶解度标签对蛋白质进行快速和并行评估（加上6xHis标记的对照载体） 。 |
| 优点： |
| ▪ Use one amplicon to simultaneously generate all your constructs；▪Rapid, simultaneous protein screening with TEV-cleavable fusion tags；▪ PCR to protein in as little as 4 days； |
| 数据： |
| Figure 1.Each pSOL vector contains everything you need to seamlessly clone in your target gene without any additional amino acids added. Fusion tags are cleavable via a TEV protease site.图1.每个pSOL载体均包含在目标基因中无缝克隆所需的一切，而无需添加任何其他氨基酸。 融合标签可通过TEV蛋白酶位点切割。Figure 2.GH1 (Human Growth Hormone) was evaluated for E. coli expression and solubility when expressed as a fusion. Fusion tags SlyD, Tsf, SUMO, Bla, and MBP demonstrated enhanced solubility (Lane S) compared to the His-control protein. T: Total Lysate, S: Cleared Soluble Fraction, I: Insoluble Pellet图2.评估GH1（人类生长激素）融合表达时的大肠杆菌表达和溶解性。 与His-control蛋白相比，SlyD，Tsf，SUMO，Bla和MBP融合标签显示出更高的溶解度（Lane S）。 T：总裂解物，S：澄清的可溶部分，I：不溶性沉淀 |
| 相关产品： |
| ▪Expresso® Solubility and Expression Screening System + Accura® High-Fidelity Polymerase（货号#49064-1） |
技术参数 产品优点 - Use one amplicon to simultaneously generate all your constructs- Rapid, simultaneous protein screening with TEV-cleavable fusion tags- PCR to protein in as little as 4 days-使用一个扩增子同时生成所有构建体 -通过TEV可切割的融合标签快速、同时进行蛋白质筛选 -只需4天即可对蛋白质进行PCR
产品应用 - The Expresso® Solubility and Expression Screening System is an all-in-one system that enables PCR amplification, rapid cloning, and expression of your protein of interest with a diverse panel of powerful and cleavable solubility tags. - Expresso®溶解度和表达筛选系统是一种多合一系统，可通过各种功能强大且可裂解的溶解度标签进行PCR扩增，快速克隆和表达您感兴趣的蛋白质。
组成成分 The Expresso Solubility and Expression Screening System contains pre-processed pSol™ vectors with 7 different fusion tags and one HIS-tagged control vector (3 reactions of each vector), positive control insert, sequencing primers, E. cloni® 10G Chemically Competent Cells (SOLOs), and 20% Rhamnose solution and 15% Glucose solution autoinduction reagents. SelecTEV™ Protease, 20% Rhamnose Solution and 15% Glucose Solution are available for purchase separately.
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