E. cloni 10G Chemically Competent Cells > 1 × 10^8 in 96-well plates，E. cloni 10G化学表达感受态细胞
货号：60096-1 ,60096-4
规格：1 plate ,4 plates
价格：5572,19635
产品类型：感受态细胞
品牌：Lucigen
| E. cloni 10G Chemically Competent Cells are a strain of E. coli that have been optimized for high
efficiency transformation by heat shock, producing > 1 x 108 cfu/µg supercoiled pUC19 DNA. These cells
are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones.They can directly replace
commonly used cloning strains like DH10B.
The E. cloni 10G strain contains inactive mcr and mrr alleles, allowing methylated genomic DNA isolated
directly from mammalian or plant cells to be cloned without deletions or rearrangements.The cells give
high yield and high quality plasmid DNA due to the endA1 and recA1 mutations.They are bacteriophage
T1-resistant (tonA mutation).The rpsL mutation confers resistance to streptomycin. |
| 优点： |
| -Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)；-Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable；-High efficiency cells result in high colony counts； |
| 相关产品： |
| ▪E. cloni 10G Chemically Competent Cells > 1 × 10^9 cfu/µg（货号#60107）▪ E. cloni 10G Chemically Competent Cells (Subcloning Grade) > 1 × 10^6 cfu/µg（货号#60108） |
技术参数 产品优点 - Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.) - Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable. - Available in a range of transformation efficiencies (1 × 106 to 1 x 109cfu/µg). - Convenient packaging options. - High efficiency cells result in high colony counts - Endorsed by SGI-DNA for Gibson Assembly® applications
组成成分 Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-18306.htm
QQ:1749072012