MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit（停产））
货号：MBCL90310
规格：10 Rxns
价格：3126
产品类型：RNA-Seq建库
品牌：Lucigen
| A MessageBOOSTER cDNA Synthesis from Cell Lysates Kit reaction amplifies, in a linear manner, the mRNA (poly[A] RNA) directly from the lysates of 1-1,000 cells, without the need for isolating total cellular RNA. The amplified RNA is converted to cDNA that can be diluted and used for qPCR. Thus, a MessageBOOSTER cDNA Synthesis from Cell Lysates Kit reaction eliminates the difficulty and uncertainty of purifying total RNA from minute samples while amplifying the mRNA for significantly improved qRT-PCR results. |
| 优点： |
| ▪ Perform RNA amplification directly from cultured cell lysates followed by cDNA synthesis▪Amplify with this high-fidelity, linear RNA amplification process that preserves the relative transcript abundance of the sample.▪ Get more data out of precious samples – use less RNA for more RT-qPCR reactions▪ Readily and reproducibly detect even low-abundance transcripts from as little as one cell.▪ No need to purify RNA: Perform RNA amplification and cDNA synthesis reactions directly from cell lysates.▪ Collect samples less often and archive cDNA for future use, saving time and effort. |
| 数据： |
| Figure 1. qPCR results from cDNA produced from a single NRK cell.cDNA was made from a single NRK cell using the MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit and qPCR of the PBGD target was performed using undiluted(red),1:10 diluted(green),1:100 diluted(blue),and 1:1,000 diluted(purple)aliguots. The low-abundance PBGD transcripts was readily detected. |
| 组成成分： |
| ▪ QuickExtract RNA Solution:储存在-20℃▪ T7-Oligo(dT) Primer A:储存在-20℃▪ RiboGuard RNase Inhibitor (40 U/µL):储存在-20℃▪ RT PreMix:储存在-20℃▪ DNA Polymerase PreMix:储存在-20℃▪ DNA Polymerase:储存在-20℃▪ cDNA Finishing Solution:储存在-20℃▪ Random Primers:储存在-20℃▪ RNase H:储存在-20℃▪ MMLV-RT:储存在-20℃▪ IVT PreMix A :储存在-20℃▪ T7 RNA Polymerase :储存在-20℃▪ 10X Transcription Reaction Buffer:储存在-20℃▪ RNase-Free DNase I (1 U/µL) :储存在-20℃▪ Forward Control PCR Primer (12.5 µM) :储存在-20℃▪ Reverse Control PCR Primer (12.5 µM):储存在-20℃▪ Nuclease-Free Water, Sterile:储存在-20℃▪ Poly(I) :储存在-20℃▪ NRK Total RNA Control (50 ng/µL):储存在-80℃ |
技术参数 产品优点 - Perform RNA amplification directly from cultured cell lysates followed by cDNA synthesis
- Amplify with this high-fidelity, linear RNA amplification process that preserves the relative transcript abundance of the sample.
- Get more data out of precious samples – use less RNA for more RT-qPCR reactions
- Readily and reproducibly detect even low-abundance transcripts from as little as one cell.
- No need to purify RNA: Perform RNA amplification and cDNA synthesis reactions directly from cell lysates.
- Collect samples less often and archive cDNA for future use, saving time and effort.
产品应用 - mRNA (poly[A] RNA) directly from the lysates of 1-1,000 cells, without the need for isolating total cellular RNA.
- The amplified RNA is converted to cDNA that can be diluted and used for qPCR.
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