beta-3 Tubulin Monoclonal Antibody (2G10)

货号：MA1-118X,MA1-118

规格：20 µL,100 µg

价格：2200,5738

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

荧光染料：Purified

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1:50-1:200 (ICC)；1:50-1:200 (IF)；1:50-1:200（IHC(P)）；5 µg （IP）；1：500-1：2000 (WB) ；1：50 Flow）
免疫原性：	A synthetic peptide corresponding to amino acids 436-450 from rat neuronal specific beta-3 tubulin.

产品详细信息MA1-118 was successfully used to detect E18 Sparague Dawley primary cortical neurons.MA1-118 can be used for immunofluorescence analysis of beta III tubulin in the ectoderm derived from human embryonic stem cells.Western blot analysis of MA1-118 detects an ~50 kDa protein in neuronal-type cells. In bovine, a unknown band at ~32 kDa is also detected. MA1-118 shows specificity to beta-3 Tubulin and is non-reactive to lysates from non-neuronal cell types (e.g. HeLa cell lysate).靶标信息Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.

数据

beta-3 Tubulin Antibody (MA1-118) in IF Immunofluorescence analysis of beta-3 Tubulin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta-3 Tubulin (2G10) Mouse Monoclonal Antibody (Product # MA1-118X) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. beta-3 Tubulin Antibody (MA1-118) in Flow Flow cytometry analysis of beta-3 Tubulin (blue histogram) on human SHSY5Y neuroblast cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a beta-3 Tubulin monoclonal antibody (Product # MA1-118) at a 1:50 dilution or PBS alone (red histogram) for 1 hour at room temperature. For flow cytometry analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) was performed and 10,000 cells were acquired for each sample.

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Oxidative medicine and cellular longevity Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study.2.PLoS neglected tropical diseases Mycolactone displays anti-inflammatory effects on the nervous system.3.Investigative ophthalmology and visual science Sustained Subconjunctival Delivery of Infliximab Protects the Cornea and Retina Following Alkali Burn to the Eye. "MA1-118 was used in immunohistochemistry - frozen section to evaluate the efficacy of subconjunctival delivery of TNF-alpha antibodies using a polymer-based drug delivery system"

技术参数

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