FosmidMAX™ DNA Purification Kit FosmidMAX™DNA纯化试剂盒
货号：FMAX046
规格：1 Kit
价格：2965
产品类型：DNA提取
品牌：Lucigen
| The FosmidMAX™ DNA Purification Kit was developed for easy, reliable isolation of highquality fosmid DNA and other large plasmid DNAs. The scalable protocol is based on a modified alkaline-lysis procedure that can be used with 1.5–100 mL of culture. Consistent yields of up to 0.6, 15 or 25 μg of fosmid DNA are obtained from 1.5, 40 or 100 mL cultures of a single-copy fosmid, respectively. Up to 4 µg of fosmid DNA can be isolated from 1.5 mL of an induced CopyControl™ fosmid clone (see below). After selective precipitation steps, treatment with the RNase Mix effectively removes contaminants that degrade DNA and can complicate spectrophotometric quantitation. Other RNases or RNase blends can be used; however, they may not completely degrade contaminating RNA, resulting in inaccurate spectrophotometric readings. There is no need for columns, resins or organic extractions. The exceptionally pure fosmid DNA can be used for many applications, including sequencing, fingerprinting, PCR and preparation of shotgun libraries. The FosmidMAX Kit can also be used to isolate cosmid DNA. |
| 优点： |
| ▪ Isolate up to 0.6, 15, or 25 µg of fosmid DNA from 1.5-, 40-, or 100-ml cultures of a single-copy fosmid, respectively, with an easy to use protocol▪ Maximize yields because the FosmidMAX kit does not use columns or binding resins that reduce yields▪ Reduce costs by using only the reagents you need based on the amount of DNA being purified▪ Purify DNA from single-copy fosmid clones, induced CopyControl fosmid and cosmid clones▪ Reduce waste and use of hazardous reagents because no columns or toxic organic solvents are used |
| 组成成分： |
| ▪ RNase Mix (1 U/μL)：储存在-20℃▪ TE Buffer：储存在-20℃▪ FosmidMAX™ Solution 1：储存在-20℃▪ FosmidMAX™ Solution 2:储存在-20℃▪ FosmidMAX™ Solution 3:储存在-20℃▪ FosmidMAX™ Solution 4:储存在-20℃ |
技术参数 产品优点 - Isolate up to 0.6, 15, or 25 µg of fosmid DNA from 1.5-, 40-, or 100-ml cultures of a single-copy fosmid, respectively, with an easy to use protocol - Maximize yields because the FosmidMAX kit does not use columns or binding resins that reduce yields - Reduce costs by using only the reagents you need based on the amount of DNA being purified - Purify DNA from single-copy fosmid clones, induced CopyControl fosmid and cosmid clones - Reduce waste and use of hazardous reagents because no columns or toxic organic solvents are used
产品应用 - Pure fosmid DNA can be used for many applications, including sequencing, fingerprinting, PCR and preparation of shotgun libraries. The FosmidMAX Kit can also be used to isolate cosmid DNA.
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