beta-3 Tubulin Polyclonal Antibody

货号： PA5-81099

规格：100 µL

价格：1758

产品类型：免疫组化一抗

品牌：eBioscience

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

类型:	多抗	同型对照：
浓度：		用法：	1:300-1:10000 (ICC)；1:300-1:10000 (IF)；1:500-1:2000 (WB)
免疫原性：	A synthetic peptide corresponding to the C-terminus of the Human TUBB3/beta III Tubuli

产品详细信息This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).靶标信息The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

数据

beta-3 Tubulin Antibody (PA5-81099) in IF Immunofluorescence staining of TUBB3 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with beta-3 Tubulin Polyclonal Antibody (Product # PA5-81099) (1:1000) at 4° C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. beta-3 Tubulin Antibody (PA5-81099) in WB Western blot analysis of beta-3 Tubulin in Lane A: A549 Whole Cell Lysate (30 µg), Lane B: HepG2 Whole Cell Lysate (30 µg) . Samples were probed using a beta-3 Tubulin Polyclonal Antibody (Product # PA5-81099) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:50 kDa. Observed band size:55 kDa.

二抗荧光二抗免疫组化一抗标签抗体

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