HI-Control® BL21(DE3) Chem Comp Cells
货号:60435-1
规格:12 rxns (SOLOs)
价格:2646
产品类型:质粒
品牌:Lucigen
HI-Control 10G cells are an E. coli strain optimized for high efficiency transformation. They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA due to the endA1 mutation. HI-Control 10G cells harbor a single-copy BAC plasmid carrying an engineered lacIq1 repressor allele. The lacIq1 allele expresses approximately 170-fold more Lac repressor than does the wildtype lacI gene . The HI-Control 10G strain does not contain T7 RNA polymerase, and the excess lac repressor in this strain further minimizes any background transcription by the bacterial polymerase. |
优点: |
▪Inducehigh level protein expressionfromlacbased promoters (T7, T5-lac, tac, trc,lac);▪Tightly regulateexpression from T7 promoters with HI-Control BL21(DE3) Chemically Competent Cells;▪ Clone targetsandreduce background protein expression with HI-Control 10G Chemically Competent Cells; |
相关产品: |
▪HI-Control™ 10G Chemically Competent Cells (SOLOs)(货号#60110-1) |
技术参数 产品优点 - Induce high level protein expression from lac based promoters (T7, T5-lac, tac, trc, lac)- Tightly regulate expression from T7 promoters with HI-Control BL21(DE3) Chemically Competent Cells- Clone targets and reduce background protein expression with HI-Control 10G Chemically Competent Cells
组成成分 Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
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