GAPDH Loading Control Monoclonal Antibody (GA1R), Biotin/GAPDH抗体

货号：MA5-15738-BTIN

规格：50 µL

价格：2753

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：Biotin

点击Invitrogen内参抗体集，查看更多内参抗体

类型:	单抗	同型对照：	IgG1
免疫原性：	Recombinant GAPDH	用法：	1:20-1:200(ICC);1:20-1:200(IF);1:50-1:500(IHC(P));1:500-1000(WB)

Product Specific InformationMA5-15738-BTIN has successfully been used for Western blot, FACS, ELISA, IHC (P) and ICC/IF applications.Background/Target InformationGAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

GAPDH Loading Control Antibody (MA5-15738-BTIN) in WBWestern blot analysis of GAPDH was performed by loading 20 µg of THP-1 whole cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk in TBST. The membrane was probed with a GAPDH loading control monoclonal antibody (Product # MA5-15738) at a dilution of 1:5000 overnight at 4°C, washed in TBST, and probed with a HRP-conjugated goat anti-mouse IgG at a dilution of 1:40,000 for 1 hr at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program. GAPDH Loading Control Antibody (MA5-15738-BTIN) in IFImmunofluorescent analysis of GAPDH (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GAPDH loading control antibody (Product # MA5-15738) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. GAPDH Loading Control Antibody (MA5-15738-BTIN) in IFImmunofluorescent analysis of GAPDH (green) showing staining in the in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GAPDH loading control antibody (Product # MA5-15738) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. GAPDH Loading Control Antibody (MA5-15738-BTIN) in FlowFlow cytometry analysis of GAPDH in NIH-3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a GAPDH loading control antibody (Product # MA5-15738) at a dilution of 2 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

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参考文献：

1.Cell death and diseaseEstradiol signaling mediates gender difference in visceral adiposity via autophagy.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.Scientific reportsLow Frequency Magnetic Fields Induce Autophagy-associated Cell Death in Lung Cancer through miR-486-mediated Inhibition of Akt/mTOR Signaling Pathway.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3.Journal of virologyProtein-Protein Interactions Suggest Novel Activities of Human Cytomegalovirus Tegument Protein pUL103.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4.Nature communicationsLegionella suppresses the host unfolded protein response via multiple mechanisms.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5.Nucleic acids researchThe amino-terminal tails of histones H2A and H3 coordinate efficient base excision repair, DNA damage signaling and postreplication repair in Saccharomyces cerevisiae.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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