TransforMax™ EC100D™ pir-116 Electrocompetent E. coli，TransforMax™ EC100D™ pir-116大肠杆菌菌株感受态细胞
货号：EC6P095H
规格：10 rxns
价格：4293
产品类型：感受态细胞
品牌：Lucigen
| The TransforMax™ EC100D™ pir+ and the TransforMax™ EC100D™ pir-116 Electrocompetent E. coli each express the Π protein (pir gene product) for replication of vectors containing the R6Kγ conditional origin of replication (R6Kγori). The cells are derived from Lucigen’s TransforMax EC100™ E. coli by P1 phage transduction with a strain containing either the pir or pir-116 gene linked to a dihydrofolate reductase (DHFR) marker. Both types of cells can be used for “rescue cloning” of genomic DNA fragments transposed with an EZ-Tn5™ <R6Kγori /KAN-2>Transposon using either the EZ-Tn5 <R6Kgori /KAN-2> Insertion Kit or the EZ-Tn5 <R6Kgori/KAN-2>Tnp Transposome Kit. The TransforMax EC100D pir+ Electrocompetent E. coli will maintain R6Kγori-containing vectors at approximately 15 copies per cell1 for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir-116 Electrocompetent E. coli are for high copy number propagation of up to 250 rescue plasmid copies per cell. |
| 优点： |
| ▪ Rescue transposition mutants created using the EZ-Tn5™ R6KɣoriKan-2> Insertion Kit or Tnp Transposome™ Kit ▪ Propagate plasmids tagged with the EZ-Tn5™ R6KɣoriKan-2> Insertion Kit |
| 相关产品： |
| ▪ EZ-Tn5<R6Kyori/KAN-2>Insertion Kit:(#EZI011RK）▪ EZ-Tn5<R6Kyori/KAN-2>Trip Tansposome Kit:(#TSM08KR） |
| 参考文献： |
| 1. Metcalf, W.W. et al., (1994) Gene 138, 1. 2. Hanahan, D. (1983) J. Mol. Biol., 166, 557. |
技术参数 产品优点 - Rescue transposition mutants created using the EZ-Tn5™ R6KɣoriKan-2> Insertion Kit or Tnp Transposome™ Kit - Propagate plasmids tagged with the EZ-Tn5™ R6KɣoriKan-2> Insertion Kit
产品应用 - Used for “rescue cloning” of genomic DNA fragments transposed with an EZ-Tn5™ <R6Kγori /KAN-2>Transposon using either the EZ-Tn5 <R6Kgori /KAN-2> Insertion Kit or the EZ-Tn5 <R6Kgori/KAN-2>Tnp Transposome Kit.
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