TransforMax™ Ec100D™ pir+ Electrocompetent E. coli/TransforMax™ Ec100D™ pir+电感受态大肠杆菌
货号：ECP09500
规格：10 rxns
价格：4293
产品类型：感受态细胞
品牌：Lucigen
| The TransforMax™ EC100D™ pir+ and the TransforMax™ EC100D™ pir-116 Electrocompetent E. coli each express the Π protein (pir gene product) for replication of vectors containing the R6Kγ conditional origin of replication (R6Kγori). The cells are derived from Lucigen’s TransforMax EC100™ E. coli by P1 phage transduction with a strain containing either the pir or pir-116 gene linked to a dihydrofolate reductase (DHFR) marker. Both types of cells can be used for “rescue cloning” of genomic DNA fragments transposed with an EZ-Tn5™ <R6Kγori /KAN-2>Transposon using either the EZ-Tn5 <R6Kgori /KAN-2> Insertion Kit or the EZ-Tn5 <R6Kgori/KAN-2>Tnp Transposome Kit. The TransforMax EC100D pir+ Electrocompetent E. coli will maintain R6Kγori-containing vectors at approximately 15 copies per cell1 for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir-116 Electrocompetent E. coli are for high copy number propagation of up to 250 rescue plasmid copies per cell.TransforMax™EC100D™pir +和TransforMax™EC100D™pir-116电感受态大肠杆菌各自表达Π蛋白（pir基因产物），用于复制包含R6Kγ条件复制起点（R6Kγori）的载体。 这些细胞是由Lucigen的TransforMax EC100™大肠杆菌通过P1噬菌体转导而产生的，该菌株含有与二氢叶酸还原酶（DHFR）标记相连的pir或pir-116基因。Z-Tn5™<R6Kγori/ KAN-2>转座子转座的基因组DNA片段的“拯救克隆”。 Tn5 <R6Kgori / KAN-2> Tnp转座体试剂盒。 TransforMax EC100D pir +电动感受态大肠杆菌将保持含R6Kγori的载体每细胞约15个拷贝1，以克隆潜在的有毒或不稳定的DNA序列。TransforMax EC100D pir-116 Electrocompetent大肠杆菌用于每个细胞最多250个营救质粒拷贝的高拷贝数繁殖。 |
| 优点： |
| ▪ Rescue transposition mutants created using the EZ-Tn5™ R6KɣoriKan-2> Insertion Kit or Tnp Transposome™ Kit ▪ Propagate plasmids tagged with the EZ-Tn5™ R6KɣoriKan-2> Insertion Kit ▪ 使用EZ-Tn5™R6KɣoriKan-2>插入试剂盒创建的拯救转座突变体或Tnp Transposome™试剂盒 ▪ 传播用EZ-Tn5™R6KɣoriKan-2>插入试剂盒标记的质粒 |
| 相关产品： |
| ▪ EZ-Tn5<R6Kyori/KAN-2>Insertion Kit:(#EZI011RK)▪ EZ-Tn5<R6Kyori/KAN-2>Trip Tansposome Kit:(#TSM08KR) |
| 参考文献： |
| 1. Metcalf, W.W. et al., (1994) Gene 138, 1. 2. Hanahan, D. (1983) J. Mol. Biol., 166, 557. |
技术参数 产品优点 - Rescue transposition mutants created using the EZ-Tn5™ R6KɣoriKan-2> Insertion Kit or Tnp Transposome™ Kit - Propagate plasmids tagged with the EZ-Tn5™ R6KɣoriKan-2> Insertion Kit- 使用EZ-Tn5™R6KɣoriKan-2>插入试剂盒或Tnp Transposome™试剂盒创建的拯救转座突变体- 传播用EZ-Tn5™R6KɣoriKan-2>插入试剂盒标记的质粒
产品应用 - Used for “rescue cloning” of genomic DNA fragments transposed with an EZ-Tn5™ <R6Kγori /KAN-2>Transposon using either the EZ-Tn5 <R6Kgori /KAN-2> Insertion Kit or the EZ-Tn5 <R6Kgori/KAN-2>Tnp Transposome Kit. - 用于使用EZ-Tn5 <R6Kgori / KAN-2>插入试剂盒或EZ-Tn5 <R6Kgori / KAN- 2> Tnp转座试剂盒。
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