TransforMax™ EPI300™-T1R Electrocompetent E. coli/TransforMax™ EPI300™-T1R感受态大肠杆菌
货号：EC02T110
规格：20 rxns
价格：6064
产品类型：感受态细胞
品牌：Lucigen
| Phage T1-Resistant TransforMax™ EPI300™-T1R Electrocompetent E. coli have been specifically engineered for use with Epicentre’s CopyControl™ Cloning Systems. The cells contain the trfA gene, whose protein product is required for initiation of replication from the oriV origin of replication contained on the CopyControl vectors. The trfA gene is under tightly regulated control of an inducible promoter. When grown on standard LB plates or in LB or SOC culture medium, expression of the trfA gene is repressed. Addition of the CopyControl Induction Solution (provided) induces expression of the trfA gene and subsequent utilization of the oriV origin of replication and high copy amplification of the CopyControl clones. In addition, these cells are resistant to bacteriophage T1 and T5 infections (tonA genotype). |
| 优点： |
| • trfA gene under tightly regulated control of an inducible promoter for copy number control of CopyControl clones. • tonA for resistance to bacteriophage T1 and T5 infections. • Supports blue/white screening of vectors. ▪Readily accepts large DNAs for construction of large-insert libraries. ▪Restriction minus [mcrA ∆(mrr-hsdRMS-mcrBC)] for efficient cloning of methylated (e.g. mammalian genomic) DNA. • Endonuclease minus (endA1) to ensure high yields of plasmid clones.▪ Recombination minus (recA1) to ensure the stability of large cloned inserts. |
技术参数 产品优点 - trfA gene under tightly regulated control of an inducible promoter for copy number control of CopyControl clones. - tonA for resistance to bacteriophage T1 and T5 infections. • Supports blue/white screening of vectors. - Readily accepts large DNAs for construction of large-insert libraries. - Restriction minus [mcrA ∆(mrr-hsdRMS-mcrBC)] for efficient cloning of methylated (e.g. mammalian genomic) DNA. - Endonuclease minus (endA1) to ensure high yields of plasmid clones. - Recombination minus (recA1) to ensure the stability of large cloned inserts.
产品应用 - Avoid phage contamination with tonA mutation for resistance to bacteriophages T1 and T5. - Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA. - Generate clones with inducible copy number using CopyControl™ vectors. - Achieve high transformation efficiencies: >1 × 1010 cfu/µg pUC19 DNA
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-18395.htm
QQ:1749072012