CD8a Monoclonal Antibody (53-6.7), PE-Cyanine5
货号：15-0081-81,15-0081-82,15-0081-83
规格：50μg,100μg,500μg
价格：1363,2128,3645
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：PE-Cy5
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5 |
| 浓度： | 0.2 mg/mL | 用法： | 0.125 μg/test (Flow)； |
| 产品详细信息Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: The 53-6.7 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocyte or splenocyte suspensions. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. |
| 数据 |
| CD8a Antibody (15-0081-82) in FlowStaining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of Anti-Mouse CD8a PE-Cyanine5 (filled histogram).Total cells were used for analysis. |
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| 参考文献： |
| 1.Journal of immunology (Baltimore, Md. : 1950)Regulatory T cells shape the resident memory T cell response to virus infection in the tissues."15-0081 was used in Flow cytometry/Cell sorting to demonstrate that Treg-dependent production of TGF-β results in increased expression of CD103 on CD8(+) T cells."AuthorsGraham JB,Da Costa A,Lund JM2.Immunology and cell biologySynergistic antitumor effects of CpG oligodeoxynucleotide and STAT3 inhibitory agent JSI-124 in a mouse melanoma tumor model."15-0081 was used in Flow cytometry/Cell sorting to provide the proof-of-principle for combining CpG and JSI-124 to enhance anti-tumour immune responses."3.Frontiers in physiologyMacrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung."Published figure using CD8a monoclonal antibody (Product # 15-0081-82) in Flow Cytometry"4.Investigative ophthalmology & visual scienceSuppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2.5.CirculationMyocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. |
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