E. cloni 5-alpha Chemically Competent Cells >1 × 10^8 cfu/µg DNA

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E. cloni 5-alpha Chemically Competent Cells >1 × 10^8 cfu/µg DNA

货号:60602-1,60602-2

规格:12 rxns (DUOs),24 rxns (DUOs)

价格:1352,2342

产品类型:质粒

品牌:Lucigen

E. cloni 5-alpha Chemically Competent Cells are versatile and useful in a wide variety of applications including routine cloning, subcloning, and plasmid isolation with or without blue/white screening. They can directly replace commonly used cloning strains like DH5α. They give high yield and high quality plasmid DNA due to the endA1 and recA1 mutations. They contain the wildtype mcr and mrr alleles, so they are NOT recommended for direct cloning of methylated genomic DNA; instead, Lucigen’s E. cloni 10G Competent Cells should be used in these cases.
优点:
▪Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.);▪Optimized genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.
相关产品:
▪Recovery Medium(货号#80026-1)

技术参数

产品优点 - Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.). - Optimized genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.

产品应用 - Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.

组成成分 Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

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