Phospho-GATA4 (Ser105) Polyclonal Antibody
货号：44-948
规格：100 µL
价格：4809
产品类型：免疫组化一抗
品牌：eBioscience
物种：人/小鼠
宿主：兔
抗体亚型：IgG
荧光染料：Purified
类型: | 多抗 | 同型对照： | |
浓度： | | 用法： | 1：10-1：50（IHC (P)）；1：500 (WB) |
免疫原性： | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human GATA-4 that contains serine 105 |
靶标信息GATA binding factor 4 (GATA4) is a GATA family transcription regulator involved in embryonic development. In addition, members of this family recognize the GATA motif which is present in the promoters of many genes. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.
数据 |
| Phospho-GATA4 (Ser105) Antibody (44-948) in IHC (P) Immunohistochemistry analysis of GATA 4 (PS105) showing staining in the nucleus of paraffin-embedded mouse lymph node (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GATA 4 (PS105) Rabbit Polyclonal Antibody (Product # 44-948) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Phospho-GATA4 (Ser105) Antibody (44-948) in WB GATA-4 (pS105) phosphospecific antibody. Cardiomyocytes overexpressing a GATA-4 Ser 105 --> Ala mutant (S105A) (1), or wild type GATA-4 (2) were stimulated with PE then immunoblotted with anti-GATA-4 (pS105). In lane 3, immunoblotting followed prior incubation with GATA-4 (pS105) phosphopeptide. Mutant analysis (1) and peptide competition (3) demonstrate antibody phospho-site specificity. |
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| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
| 1.Scientific reports Cardiac Actions of a Small Molecule Inhibitor Targeting GATA4-NKX2-5 Interaction.2.The Journal of cell biology Nuclear-localized focal adhesion kinase regulates inflammatory VCAM-1 expression. |
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