GATA4 Monoclonal Antibody (6H10)

货号：MA5-15532

规格：100 µL

价格：5845

产品类型：免疫组化一抗

品牌：eBioscience

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG2b, kappa

荧光染料：Purified

类型:	单抗	同型对照：
浓度：		用法：	1-3µL (ChIP)；1:500-1:2000 (WB)
免疫原性：	Purified recombinant fragment of human GATA4 expressed in E. Coli.

产品详细信息MA5-15532 targets GATA4 in WB and ChIP applications and shows reactivity with Human and mouse samples.The MA5-15532 immunogen is purified recombinant fragment of human GATA4 expressed in E. Coli.MA5-15532 detects GATA4 which has a predicted molecular weight of approximately 46kDa.靶标信息This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.

数据

GATA4 Antibody (MA5-15532) in WB Western blot analysis of GATA4 using GATA4 monoclonal antibody (Product # MA5-15532) in rat fetal heart (1) and adult heart (2) tissues lysate. GATA4 Antibody (MA5-15532) in ChIP Chromatin immunoprecipitation analysis of GATA4 was performed using cross-linked chromatin from 1x10^6 HCT116 colon carcinoma cells treated with serum for 0, 15, and 30 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of a GATA4 monoclonal antibody (Product # MA5-15532). Chromatin aliquots from ~1x10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

二抗荧光二抗免疫组化一抗标签抗体

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技术参数

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