Gata-4 Monoclonal Antibody (eBioEvan), eBioscience™

货号：14-9980-82 ,14-9980-80

规格：100 µg,25 µg

价格：3794,1575

产品类型：免疫组化一抗

品牌：eBioscience

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

荧光染料：Purified

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	Assay Dependent（IHC (P)）；1:1000 (WB)
免疫原性：

产品详细信息Description: The eBioEvan monoclonal antibody reacts with mouse and human Gata-4. In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights. The eBioEvan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4.Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers. The mouse Gata-4 gene encodes a protein of 48kDa. Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival. Gata-4 is expressed during fetal development, as well as in the adult mouse heart. It also appears to modulate gene expression in the gut, liver, and gonads.Applications Reported: This eBioEvan antibody has been reported for use in immunoblotting (WB) and immunohistochemistry.Applications Tested: This eBioEvan antibody has been tested by western blot analysis of mouse heart lysates. As a starting dilution, it is recommended to use this purified antibody at 2µg/mL for western blot analysis. The eBioEvan antibody has been tested on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

数据

Gata-4 Antibody (14-9980-82) in WB Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Ovary (Lane 2) and Mouse Brain (Lane 3). The blot was probed with Anti-GATA-4 Rat Monoclonal Antibody (Product # 14-9980-82, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # PA1-29927, 0.25 ug/ml, 1:4000 dilution). A 50 kDa band corresponding to GATA-4 was detected across the tissues tested. Additional band around 25kDa from circulating IgGs was observed in Mouse Heart and Mouse Ovary lysates but not in Mouse Brain lysate which is selectively permeable.

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

技术参数

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