CD25 Monoclonal Antibody (OX39), APC

货号：17-0390-82

规格：100μg

价格：2583

产品类型：流式抗体

品牌：Thermo Fisher

物种：大鼠

宿主：小鼠

抗体亚型：IgG

克隆号：OX39

荧光染料：APC

抗体类型:	单抗	同型对照：	IgG1, kappa
免疫原性：		用法：	0.125 µg/test(Flow）

Description: The OX39 monoclonal antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineages as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.The OX39 clone has been shown to deplete CD25 cells in vivo.Applications Reported: This OX39 antibody has been reported for use in flow cytometric analysis and immunohistochemistry.Applications Tested: This OX39 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD25 Antibody (17-0390-82) in Flow Staining of rat splenocytes with Anti-Rat CD4 FITC (Product # 11-0040-82) and 0.06 µg of Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or 0.06 µg of Anti-Rat CD25 APC (right). Total viable cells were used for analysis.

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参考文献：

1. PloS oneRecombinant HLA-G as Tolerogenic Immunomodulant in Experimental Small Bowel Transplantation."17-0390 was used in Flow cytometry/Cell sorting to examine the therapeutic approach of immunosuppression using HLA-G in solid organ transplantation, showing a significant immunosuppressive effect in a model of allogenic experimental intestinal transplantation."Authorsvon Websky MW,Kitamura K,Ludwig-Portugall I,Kurts C,von Laffert M,LeMaoult J,Carosella ED,Abu-Elmagd K,Kalff JC,Schäfer N2. American journal of physiology. Heart and circulatory physiologyTreatment with N-acetyl-seryl-aspartyl-lysyl-proline prevents experimental autoimmune myocarditis in rats."17-0390 was used in Flow cytometry/Cell sorting to examine the effects of N-acetyl-seryl-aspartyl-lysyl-proline in experimental autoimmune myocarditis, a model of T cell-mediated autoimmune disease."AuthorsNakagawa P,Liu Y,Liao TD,Chen X,González GE,Bobbitt KR,Smolarek D,Peterson EL,Kedl R,Yang XP,Rhaleb NE,Carretero OA3. BMC immunologyPeripheral blood T regulatory cell counts may not predict transplant rejection."17-0390 was used in Flow cytometry/Cell sorting to examine the frequency and cytokine profile of Foxp3+ Tregs in rat recipients of cardiac allografts following a dose of Rapamycin."AuthorsHuang Y,Shan J,Zhang C,Zhang J,Feng L,Li S,Li Y

技术参数

产品应用 Flow

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