KLF5 Recombinant Rabbit Monoclonal Antibody (23H10L5)

货号：701885

规格：100 µg

价格：4809

产品类型：免疫组化一抗

品牌：eBioscience

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-2 µg/mL (ICC)；1-2 µg/mL (IF)；2.5 µg/1x10^6 cells(ChIP)
免疫原性：	Peptides corresponding to Human KLF5 (aa 11-24, 358-372)

产品详细信息This antibody is predicted to react with Monkey, Rabbit and PigRecombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. 靶标信息KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. It contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. KLF5 is a transcription activator that promotes cell proliferation and oncogenesis.

数据

KLF5 Antibody (701885) in IF For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous KLF5 using Anti- KLF5 Recombinant Rabbit Monoclonal Antibody (Product # 701885, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of KLF5 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c demonstrating predominant nuclear localization of KLF5. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. KLF5 Antibody (701885) in ChIP Enrichment of endogenous KLF5 protein at specific gene loci using Anti-KLF. Recombinant Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-KLF5 Recombinant Rabbit Monoclonal Antibody (Product # 701885, 5 µg) on sheared chromatin from 2 million A549 cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 FastqPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the activ SOX4, KLF4 region used as positive control target gene, and the region of the inactive KLF4 UPSTRAEM, SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

二抗荧光二抗免疫组化一抗标签抗体

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