5'-DMT-T Super Column; 1000 Å
货号：SCG1-1300-1,SCG1-1300-2,SCG1-1300-5
规格：1 µmol,200 nmol,50 nmol
价格：54.67,28.01,25.19
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| 5'-DMT-T-Suc-CPG is used for the placement of T on the 3' end of oligonucleotides. The 1000 Å CPG is best suited for the synthesis of DNA sequences up to 100-mers in length. |
| 特性： |
| ▪ Appearance:White Powder▪ Extinction Coefficient at 260 nm:8400 |
| 产品用途： |
| Cleavage conditions:Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.ABI 3900 users: Columns should be compatible with standard ABI protocols. However, when using 50 nmol Super Columns, the following changes are recommended:
1) Change the head pressure/purge pressure/chamber pressure to 3.5 psi.
2) An additional oxidation step
3) OPTIONAL (but may help): extension of the coupling time to 20 sec Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is：304.19 |
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