Pierce Peptide Retention Time Calibration Mixture 肽保留时间校准混合物
货号：88321
规格：200 µL
价格：6693
产品类型：蛋白表达研究
品牌：Thermo Fisher
| The Thermo Scientific Pierce Peptide Retention Time Calibration Mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment.The Pierce Peptide Retention Time Calibration Mixture is used to optimize and assess LC parameters, identify the total peptide elution window, and optimize MS parameters. Testing the mixture frequently throughout the lifetime of a column or including it in quantitative samples makes it possible to monitor and normalize LC-MS performance between samples and over time. When used with Thermo Scientific Pinpoint 1.1 software, the calibration mixture can predict peptide retention times based upon peptide sequence or relative retention time between instruments. The mixture can also be used with Pinpoint software to establish normalized collision energies between platforms. This allows normalization of methods and eases the transfer of quantitative methods between LC-MS platforms. Pinpoint software, sample files, tutorials, and videos can be downloaded from the Thermo Proteomics Portal.The Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic heavy peptides mixed at an equimolar ratio that elute across the chromatographic gradient. The observed retention times and hydrophobicity factors (HF) for these calibrant peptides are fit to a linear equation to determine the slope of the retention time/HF relationship. This equation and the HF of an uncharacterized peptide are then used to predict retention time.The prediction of peptide retention time assists in the development of multiplexed, high throughput mass spectrometric assays. The Pierce Peptide Retention Time Calibration Mixture and Thermo Scientific Pinpoint software streamline the transition from qualitative protein discovery results to the development of targeted MS assays on Thermo Scientific triple quadrupole, Orbitrap, Exactive, and ion trap mass spectrometers. The Pierce Peptide Retention Time Calibration Mixture permits optimization and regular assessment of chromatographic performance and for rapid development of multiplexed, scheduled targeted MS assays for the quantification of dozens to hundreds of peptide targets per run. |
| 特点： |
| - Assess chromatography and MS instrument performance- Predict peptide retention across multiple instrument platforms- Predict peptide retention time from sequence using calculated hydrophobicity factor- Optimize scheduled MS acquisition windows for improved quantification and increased multiplexing- Normalize collision energies between instruments and platforms- Normalize results for variation in retention times and peak intensities between runs |
| 数据： |
Thermo Scientific Pierce Peptide Retention Time Calibration Mixture Components and PropertiesThe peptide sequences, peptide masses and chromatographic behavior of each component of the Peptide Retention Time Calibration Mixture are given above. The position and identity of the heavy lysine+ 8Da or arginine+10Da isotope-labeled amino acid in each sequence is indicated in bold. |
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