OSMR Human Uncoated ELISA Kit with Plates/人OSMR ELISA试剂盒含未包被板

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OSMR Human Uncoated ELISA Kit with Plates/人OSMR ELISA试剂盒含未包被板

货号:88-50330-86,88-50330-22

规格:10X96 Tests,2X96 Tests

价格:6537,3125

产品类型:自定义ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

312.5 pg/mL

检测范围312.5-20,000 pg/mL

样本类型/体积

血浆 10 μL

血清 10 μL

上清液 10 μL

操作时间

1 hr 45 min

测试时间

24 hr 30 min

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 X 96 Tests

组份

- 捕获抗体:Pre-titrated, 纯化抗体

- 检测抗体:Pre-titrated, Biotin偶联抗体

- 标准品:重组细胞因子,用于生成标准曲线和校准样品

- 10X包被缓冲液:用于包被捕获抗体的缓冲液

- 5XELISA/ELISPOT稀释液:封闭和稀释检测抗体和酶的缓冲液

- 酶:Pre-titrated Avidin-HRP

- 底物:1X TMB溶液

- 分析证书:特定批次说明书用于抗体和标准品稀释

- 96孔板:Corning Costar 9018(含产品目录号,后缀 -22,-76,-86)

储存温度

2-8℃

Protein nameOSMR
Protein aliasesIL-31 receptor subunit beta, IL-31R subunit beta, Interleukin-31 receptor subunit beta, oncostatin-M specific receptor beta subunit, Oncostatin-M-specific receptor subunit beta

物种(Tested)

试剂盒类型

Uncoated ELISA Kitwith Plates

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesIL-31R-beta, IL-31RB, OSMR, OSMRB, PLCA1
Gene ID(Human) 9180
Gene symbolOSMR
UniProt ID(Human) Q99650
The Human Oncostatin M Receptor (OSMR) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human OSMR. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人Oncostatin M受体(OSMR)未包被的ELISA试剂盒包含预先匹配的抗体对,板和试剂,用于进行定量酶联免疫吸附测定(ELISA),以检测和定量人类OSMR的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应,需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部,这是一整夜的过程。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Oncostatin M is a member of the IL6 family of cytokines. Functional receptors for IL6 family cytokines are multisubunit complexes involving members of the hematopoietin receptor superfamily. Many IL6 cytokines utilize gp130 as a common receptor subunit. OSM binds to the gp130 receptor subunit and, in association with the leukemia inhibitory factor receptor, induces a proliferative response in permissive cells. OSMR is an alternative subunit (for an OSM receptor complex (a heterodimer of gp130 and OSMR) that is activated by OSM but not by LIF.Oncostatin M is a member of the IL6 family of cytokines. Functional receptors for IL6 family cytokines are multisubunit complexes involving members of the hematopoietin receptor superfamily. Many IL6 cytokines utilize gp130 as a common receptor subunit. OSM binds to the gp130 receptor subunit and, in association with the leukemia inhibitory factor receptor, induces a proliferative response in permissive cells. OSMR is an alternative subunit (for an OSM receptor complex (a heterodimer of gp130 and OSMR) that is activated by OSM but not by LIF. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.抑癌素M是细胞因子IL6家族的成员。 IL6家族细胞因子的功能受体是涉及血细胞生成素受体超家族成员的多亚基复合物。许多IL6细胞因子利用gp130作为常见的受体亚基。 OSM与gp130受体亚基结合,并与白血病抑制因子受体结合,在允许的细胞中诱导增殖反应。 OSMR是OSM而非LIF激活的另一亚基(对于OSM受体复合物(gp130和OSMR的异二聚体)),抑癌素M是IL6细胞因子家族的成员,IL6家族细胞因子的功能性受体是多亚基复合体。涉及造血素受体超家族成员的细胞,许多IL6细胞因子利用gp130作为共同的受体亚基,OSM与gp130受体亚基结合,并与白血病抑制因子受体结合,在允许的细胞中诱导增殖反应。 (对于OSM而不是LIF激活的OSM受体复合物(gp130和OSMR的异源二聚体)。出版说明:该RefSeq记录包含可用于该基因的出版物的子集。请参阅Entrez基因记录以访问其他出版物。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。
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