Pierce 96-well Microdialysis Plate, 10K MWCO / 96孔微透析板,10K MWCO
货号:88260
规格:1 plate
价格:7808
产品类型:蛋白表达研究
品牌:Thermo Fisher
Thermo Scientific Pierce 96-well 10K MWCO Microdialysis Plates are automation-compatible, microplate dialysis devices for rapidly processing 1 to 96 samples with volumes 10 to 100 µL. This kit comes with 12 cartridges of eight microdialysis devices in a 96 deep-well plate.Each sample processed with the Pierce 96-well Microdialysis Dialysis Plate is dialyzed inside a single, removable dialysis device created by two regenerated cellulose membranes separated by ~2mm. The short diffusion distance and large surface area facilitates rapid dialysis with the removal of salts and small molecules often completed in 2 to 4 hours. Sample recovery is performed using standard laboratory micropipettes. Individual microdialysis devices can also be used with 2 mL microcentrifuge tubes for processing single samples. The available plate seals are made of high-quality polypropylene and help protect samples from contamination and evaporation, especially during long-term storage.Each 96-well Microdialysis Plate comes pre-loaded with twelve 8-dialysis device strips. Each dialysis device strip can be removed from the plate and individual microdialysis devices easily separated, allowing only the number of needed devices to be used and eliminating waste. The sample chamber inside each microdialysis device is formed by two low-binding regenerated cellulose membranes that are rated to retain proteins and other macromolecules >10,000 Daltons while allowing removal of buffer salts and contaminants <1000 Daltons. Surface tension and the small distance between the membranes facilitate highly efficient sample recovery using standard laboratory pipettes.Using the Microdialysis Plate, sample dialysis can be efficiently performed in a standard 96 deep-well plate with a minimal amount of buffer while still providing >95% removal of small molecules. The assembled device is compatible with standard 96-well laboratory equipment and automated liquid-handling systems making it an ideal option for high-throughput applications. Single microdialysis devices can be used independently in 2 mL microcentrifuge tube for processing single samples. |
特点: |
- High yield—greater than 90% protein recovery after dialysis- Rapid—dialysis completed in 2 to 4 hours- Easy to use—sample loading and retrieval can be done with a standard pipette- Flexible—detachable 8-device strips, scalable from 1 to 96 samples- Automation-compatible—plate format conforms to the SBS Microplate Standard |
应用: |
- Protein and DNA desalting/buffer exchange- Removal of dyes after protein labeling- Enzyme activity/binding assays- Drug binding studies (also see Rapid Equilbrium Dialysis "RED" Devices) |
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