Pierce LysN Protease, MS Grade / LysN蛋白酶，MS级
货号：90300
规格：20 µg
价格：0
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| Thermo Scientific Pierce LysN Protease, MS Grade, is highly purified native Lys-N protease that has been validated for maximum activity and stability in proteomic applications.Thermo Scientific Pierce LysN is a mass spectrometry (MS)-grade zinc metalloprotease derived from Grifola frondosa. The native Pierce LysN Protease has been highly purified to improve stability, specific activity, and cleavage selectivity. Unlike trypsin, LysN protease cleaves at the amino-terminus of lysine residues. As a result, the peptides generated by LysN are longer than those generated by trypsin and have more prevalent charged amino terminal peptide fragments. Additionally, LysN protease is more promising for epigenetic MS applications than LysC or trypsin because it is capable of cleaving methylated lysines [1]. The thermostability and chemical compatibility with denaturants make LysN protease ideal for digestion of complex protein samples for “shotgun” proteomics.LysN protease is active over a wide range of temperatures and denaturing conditions. Efficient protein digestion can be completed in 2 hours at 50°C or 4 hours at 37°C, however, digestion is possible at both lower and higher temperatures. LysN also remains active under moderate denaturing conditions including 0.1% SDS, 6M urea or heating to 70°C. Maximal LysN activity occurs at pH 7to9. The lyophilized enzyme is stable for 2 years when stored at -20°C, and reconstituted stock solutions of LysN are stable at -80°C for 2 years or -20°C for one year without significant loss in activity. |
| 特点： |
| - Thermostable – provides better digestion at higher temperatures (e.g., 50°C) in less time- Complementary to tryptic digests – cleaves the opposite side of lysine from trypsin- High purity – no additional (off-target) protease activity detected- N-terminal lysine cleavage specificity – at least 90% for a complex protein sample- Versatile – enzyme is effective at many temperatures and in denaturing conditions- Stable – provided in a lyophilized format |
| 应用： |
| - Improved sequence coverage of protein digests- De novo sequencing with collision induced dissociation (CID)- Epigenetic studies- In-solution digestion of proteins |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-18975.htm
QQ:1749072012